How to cite this article: Adebayo O, Hookway TA, Hu JZ, Billiar KL, Rolle MW. 2013. Self-assembled smooth muscle cell tissue rings exhibit greater tensile strength than cell-seeded fibrin or collagen gel rings. J Biomed Mater Res Part A 2013:101A:428–437.
Self-assembled smooth muscle cell tissue rings exhibit greater tensile strength than cell-seeded fibrin or collagen gel rings †
Article first published online: 3 AUG 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 2, pages 428–437, February 2013
How to Cite
Adebayo, O., Hookway, T. A., Hu, J. Z., Billiar, K. L. and Rolle, M. W. (2013), Self-assembled smooth muscle cell tissue rings exhibit greater tensile strength than cell-seeded fibrin or collagen gel rings . J. Biomed. Mater. Res., 101A: 428–437. doi: 10.1002/jbm.a.34341
- Issue published online: 18 DEC 2012
- Article first published online: 3 AUG 2012
- Manuscript Accepted: 2 JUL 2012
- Manuscript Revised: 25 MAY 2012
- Manuscript Received: 26 MAY 2011
- National Science Foundation. Grant Number: REU EEC 0754996
- National Institutes of Health. Grant Number: R15 HL097332
- fibrin gel;
- collagen gel;
- cell-derived matrix;
- vascular tissue engineering;
- soft tissue biomechanics
In this study, we created self-assembled smooth muscle cell (SMC) tissue rings (comprised entirely of cells and cell-derived matrix; CDM) and compared their structure and material properties with tissue rings created from SMC-seeded fibrin or collagen gels. All tissue rings were cultured statically for 7 days in supplemented growth medium (with ε-amino caproic acid, ascorbic acid, and insulin-transferrin-selenium), prior to uniaxial tensile testing and histology. Self-assembled CDM rings exhibited ultimate tensile strength and stiffness values that were two-fold higher than fibrin gel and collagen gel rings. Tensile testing of CDM, fibrin gel and collagen gel rings treated with deionized water to lyse cells showed little to no change in mechanical properties relative to untreated ring samples, indicating that the ECM dominates the measured ring mechanics. In addition, CDM rings cultured in supplemented growth medium were significantly stronger than CDM rings cultured in standard, unsupplemented growth medium. These results illustrate the potential utility of self-assembled cell rings as model CDM constructs for tissue engineering and biomechanical analysis of ECM material properties. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.