How to cite this article: Zhou D, Xiong L, Wu Q, Guo R, Zhou Z, Zhu Q, Jiang Y, Huang J. 2013. Effects of transmyocardial jet revascularization with chitosan hydrogel on channel patency and angiogenesis in canine infarcted hearts. J Biomed Mater Res Part A 2013:101A:567–574.
Effects of transmyocardial jet revascularization with chitosan hydrogel on channel patency and angiogenesis in canine infarcted hearts†
Article first published online: 4 SEP 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 2, pages 567–574, February 2013
How to Cite
Zhou, D., Xiong, L., Wu, Q., Guo, R., Zhou, Z., Zhu, Q., Jiang, Y. and Huang, J. (2013), Effects of transmyocardial jet revascularization with chitosan hydrogel on channel patency and angiogenesis in canine infarcted hearts. J. Biomed. Mater. Res., 101A: 567–574. doi: 10.1002/jbm.a.34346
- Issue published online: 18 DEC 2012
- Article first published online: 4 SEP 2012
- Manuscript Accepted: 26 JUN 2012
- Manuscript Revised: 23 JUN 2012
- Manuscript Received: 20 APR 2012
- National Natural Science Foundation of China. Grant Numbers: 30527001, 30670870
- Foundation for Science and Technology Research Project of Chongqing. Grant Numbers: CSTS2005AA5008-5, CSTS2009AB5003
- myocardial infarction;
- transmyocardial revascularization;
- needle-free injector;
- growth factors
The purpose of this study was to investigate whether transmyocardial jet revascularization (TMJR) with chitosan scaffolds retains channel patency and enhances angiogenesis after acute myocardial infarction (AMI) in a canine model. A total of 32 canines were randomly divided into four groups: myocardial infarction (MI), normal saline (NS), chitosan hydrogel (CH), and chitosan plus growth factor (CH + GF) groups. TMJR was performed surgically using a needle-free injector from the epicardium of canines in the NS, CH, and CH + GF groups; channels were filled with NS, CH, and CH + GF, respectively. After 6 weeks, the patency of the channels and angiogenesis around the channels were assessed by hematoxylin–eosin staining, immunohistochemistry, and Masson's trichrome staining. Results suggest that the channels in the CH and CH + GF groups may retain patency with luminal endothelization. Moreover, the vessel densities of the NS, CH, and CH + GF groups were significantly higher than that of the MI group, and that of the CH + GF group was the highest (p < 0.05). This study suggests that TMJR with chitosan scaffolds may help retain transmyocardial channel patency and enhance angiogenesis after AMI in canines. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.