How to cite this article: Gerhardt L-C, Widdows KL, Erol MM, Nandakumar A, Roqan IS, Ansari T, Boccaccini AR. 2013. Neocellularization and neovascularization of nanosized bioactive glass-coated decellularized trabecular bone scaffolds. J Biomed Mater Res Part A 2013:101A:827–841.
Article first published online: 11 SEP 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 3, pages 827–841, March 2013
How to Cite
Gerhardt, L.-C., Widdows, K. L., Erol, M. M., Nandakumar, A., Roqan, I. S., Ansari, T. and Boccaccini, A.R. (2013), Neocellularization and neovascularization of nanosized bioactive glass-coated decellularized trabecular bone scaffolds. J. Biomed. Mater. Res., 101A: 827–841. doi: 10.1002/jbm.a.34373
These authors contributed equally to this work.
- Issue published online: 25 JAN 2013
- Article first published online: 11 SEP 2012
- Manuscript Accepted: 2 JUL 2012
- Manuscript Revised: 2 JUN 2012
- Manuscript Received: 20 MAR 2012
- Partial funding by King Abdullah University of Science and Technology (KAUST; 2009–2010)
- decellularized bone;
- bioactive glass;
- vascular endothelial growth factor;
In this study, the in vivo recellularization and neovascularization of nanosized bioactive glass (n-BG)-coated decellularized trabecular bone scaffolds were studied in a rat model and quantified using stereological analyses. Based on the highest amount of vascular endothelial growth factor (VEGF) secreted by human fibroblasts grown on n-BG coatings (0–1.245 mg/cm2), decellularized trabecular bone samples (porosity: 43–81%) were coated with n-BG particles. Grown on n-BG particles at a coating density of 0.263 mg/cm2, human fibroblasts produced 4.3 times more VEGF than on uncoated controls. After 8 weeks of implantation in Sprague–Dawley rats, both uncoated and n-BG-coated samples were well infiltrated with newly formed tissue (47–48%) and blood vessels (3–4%). No significant differences were found in cellularization and vascularization between uncoated bone scaffolds and n-BG-coated scaffolds. This finding indicates that the decellularized bone itself may exhibit growth-promoting properties induced by the highly interconnected pore microarchitecture and/or proteins left behind on decellularized scaffolds. Even if we did not find proangiogenic effects in n-BG-coated bone scaffolds, a bioactive coating is considered to be beneficial to impart osteoinductive and osteoconductive properties to decellularized bone. n-BG-coated bone grafts have thus high clinical potential for the regeneration of complex tissue defects given their ability for recellularization and neovascularization. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 827–841, 2013.