Get access

Cell adhesion and osteogenic differentiation on three-dimensional pillar surfaces

Authors

  • Emilia Kaivosoja,

    1. Department of Medicine, Institute of Clinical Medicine, Helsinki University Central Hospital, Helsinki, Finland
    2. Department of Electronics, School of Electrical Engineering, Aalto University, Espoo, Finland
    Search for more papers by this author
  • Pia Suvanto,

    1. Department of Materials Science and Engineering, School of Chemical Technology, Aalto University, Espoo, Finland
    Search for more papers by this author
  • Gonçalo Barreto,

    1. Department of Medicine, Institute of Clinical Medicine, Helsinki University Central Hospital, Helsinki, Finland
    Search for more papers by this author
  • Susanna Aura,

    1. Department of Materials Science and Engineering, School of Chemical Technology, Aalto University, Espoo, Finland
    Search for more papers by this author
  • Antti Soininen,

    1. ORTON Research Institute of the ORTON Orthopaedic Hospital, Helsinki, Finland
    Search for more papers by this author
  • Sami Franssila,

    1. Department of Materials Science and Engineering, School of Chemical Technology, Aalto University, Espoo, Finland
    Search for more papers by this author
  • Yrjö T. Konttinen

    Corresponding author
    1. Department of Medicine, Institute of Clinical Medicine, Helsinki University Central Hospital, Helsinki, Finland
    2. ORTON Research Institute of the ORTON Orthopaedic Hospital, Helsinki, Finland
    3. COXA Hospital for Joint Replacement, Tampere, Finland
    • Department of Medicine, Institute of Clinical Medicine, Helsinki University Central Hospital, Helsinki, Finland
    Search for more papers by this author

  • How to cite this article: Kaivosoja E, Suvanto P, Barreto G, Aura S, Soininen A, Franssila S, Konttinen YT. 2013. Cell adhesion and osteogenic differentiation on three-dimensional pillar surfaces. J Biomed Mater Res Part A 2013:101A:842–852.

Abstract

We hypothesized that when compared with conventional two-dimensional (2D) cultures, substrates containing 3D micropillars would allow cells to grow at levels, activating their cytoskeleton to promote osteogenesis. Fibroblasts, osteoblast-like cells, and mesenchymal stem cells (MSCs) were studied. Planar substrates were compared with 200-nm-, 5-μm-, and 20-μm-high pillars of Ormocomp®, Si, diamond-like carbon, or TiO2. Scanning electron microscopy and staining of actin cytoskeleton showed 7.5-h adhesion to pillar edges and 5-day stretching between adhesion contacts > 100-μm distances of fibroblast and MSC in 3D networks, whereas SaOS-2 cells adhered flatly and individually on horizontal and vertical surfaces. ERK and ROCK immunostaining at 14 and 21 days confirmed activation of the cytoskeleton. In contrast to expectations, success to induce osteogenesis was dominated by the cytocompatibility of the substrate over the 3D structure. This was shown using early alkaline phosphatase, intermediate osteopontin, and late mineralization markers, together with bone nodule formation, which were seen in planar substrates and low-profile TiO2 pillars, but were poor in the 20-μm landscape. The lack of intercellular contacts seems to halt the osteogenesis-promoting effects of cytoskeletal organization and tension described earlier. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 842–852, 2013.

Ancillary