How to cite this article: de Mulder ELW, Hannink G, Koens MJW, Löwik DWPM, Verdonschot N, Buma P. 2013. Characterization of polyurethane scaffold surface functionalization with diamines and heparin. J Biomed Mater Res Part A 2013:101A:919–922.
Characterization of polyurethane scaffold surface functionalization with diamines and heparin†
Article first published online: 8 SEP 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 4, pages 919–922, April 2013
How to Cite
de Mulder, E. L. W., Hannink, G., Koens, M. J. W., Löwik, D. W. P. M., Verdonschot, N. and Buma, P. (2013), Characterization of polyurethane scaffold surface functionalization with diamines and heparin. J. Biomed. Mater. Res., 101A: 919–922. doi: 10.1002/jbm.a.34389
- Issue published online: 21 FEB 2013
- Article first published online: 8 SEP 2012
- Manuscript Accepted: 24 JUL 2012
- Manuscript Revised: 25 JUN 2012
- Manuscript Received: 9 MAY 2012
- Netherlands Institute for Regenerative Medicine (NIRM)
- The Dutch Arthritis Foundation (Reumafonds)
- tissue engineering;
- surface treatment;
Polyurethane scaffolds (PUs) have a good biocompatibility but lack cell recognition sites. In this study, we functionalized the surface of a PU, P(D/L)LA and PCL (50:50) containing urethane segments, with heparin. The first step in this functionalization, aminolysis, lead to free amine groups on the surface of the PU. Free amine content was determined to be 6.4 nmol/mL/mg scaffold, a significant increase of 230%. Subsequently, heparin was crosslinked. Immunohistochemistry demonstrated the presence of heparin homogeneous throughout the 3D porous scaffold. Young's modulus decreased significantly till 50% of the native stiffness after aminolysis and did not change after heparin crosslinking. Contact angle on PU films significantly decreased from 82.7° to 64.3° after heparin crosslinking, indicating a more hydrophilic surface. This functionalization beholds great potential for tissue engineering purposes. When used in a load-bearing environment, caution is necessary due to reduction in mechanical stiffness. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.