These authors contributed equally to this work.
Calcium phosphate cements loaded with basic fibroblast growth factor: Delivery and in vitro cell response†
Article first published online: 8 SEP 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 4, pages 923–931, April 2013
How to Cite
Perez, R. A., Kim, T.-H., Kim, M., Jang, J.-H., Ginebra, M.-P. and Kim, H.-W. (2013), Calcium phosphate cements loaded with basic fibroblast growth factor: Delivery and in vitro cell response. J. Biomed. Mater. Res., 101A: 923–931. doi: 10.1002/jbm.a.34390
How to cite this article: Perez RA, Kim T-H, Kim M, Jang J-H, Ginebra M-P, Kim H-W. 2013. Calcium phosphate cements loaded with basic fibroblast growth factor: Delivery and in vitro cell response. J Biomed Mater Res Part A 2013:101A:923–931.
- Issue published online: 21 FEB 2013
- Article first published online: 8 SEP 2012
- Manuscript Accepted: 24 JUL 2012
- Manuscript Revised: 10 JUL 2012
- Manuscript Received: 4 MAY 2012
- Priority Research Centers Program. Grant Number: 2009-0093829
- WCU program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, South Korea. Grant Number: R31-10069
- calcium phosphate cements;
- powder size;
- growth factor delivery;
- cell proliferation;
- bone regeneration
Combining calcium phosphate cements (CPCs) with bioactive molecules improves their bone regeneration potential. Although CPCs are highly osteoconductive, sometimes they have limited biological responses, especially in terms of cell proliferation. Here, we used basic fibroblast growth factor (bFGF) in an α-tricalcium phosphate cement with different initial powder sizes (coarse vs. fine; designated as CPC-C and CPC-F, respectively) and investigated the behavior of bFGF loading and release, as well as the effects on osteoblast responses. bFGF was loaded at 10 μg/ml or 25 μg/ml onto the set form of two types of CPCs, aiming to allow penetration into the pore structure and adsorption onto the cement crystallites. The CPC formulated with fine powder (CPC-F) had higher specific surface area and smaller-sized pores and retained slightly higher amounts of bFGF within the structure. The bFGF release study performed for 3 weeks showed a sustained-release profile; after an initial rapid release over approximately 3 days, further release pattern was almost linear. Compared to CPC-F, CPC-C showed a much faster release pattern. The effects of the bFGF incorporation within CPCs on cellular responses were assessed in terms of cell proliferation using MC3T3-E1 pre-osteoblastic cells. Compared with bFGF-free CPCs (both CPC-C and CPC-F), those containing bFGF stimulated cell proliferation for up to 7 days. An inhibition study of bFGF receptor demonstrated that the improvement of cell proliferation resulted from the role of bFGF released from the CPCs. This study provides beneficial information on improving the biological properties of CPCs by combining them with specific therapeutic molecules, and particularly with bFGF, showing that the cell proliferative ability was significantly stimulated, which may have potential applications for further use in stem cell-based bone tissue engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.