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Generation of osteogenic construct using periosteal-derived osteoblasts and polydioxanone/pluronic F127 scaffold with periosteal-derived CD146 positive endothelial-like cells

Authors

  • Jin Ho Lee,

    1. Department of Advanced Materials, College of Life Science and Nano Technology, Hannam University, Yuseong-gu, Daejeon 305-811, South Korea
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    • These authors contributed equally to this work.

  • Shin-Won Kim,

    1. Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Mulgeum-eup, Yangsan 626-770, South Korea
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    • These authors contributed equally to this work.

  • Uk-Kyu Kim,

    1. Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Mulgeum-eup, Yangsan 626-770, South Korea
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  • Se Heang Oh,

    1. Department of Advanced Materials, College of Life Science and Nano Technology, Hannam University, Yuseong-gu, Daejeon 305-811, South Korea
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  • Sang June-Kim,

    1. Department of Advanced Materials, College of Life Science and Nano Technology, Hannam University, Yuseong-gu, Daejeon 305-811, South Korea
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  • Bong-Wook Park,

    1. Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Biomedical center (BK21), Gyeongsang National University School of Medicine, Chilam-dong, Jinju 660-702, South Korea
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  • Jin-Hyun Kim,

    1. Clinical Research Institute of Gyeongsang National University Hospital, Chilam-dong, Jinju 660-702, South Korea
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  • Young-Sool Hah,

    1. Clinical Research Institute of Gyeongsang National University Hospital, Chilam-dong, Jinju 660-702, South Korea
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  • Deok Ryong Kim,

    1. Department of Biochemistry, Institute of Health Sciences, Biomedical center (BK21), Gyeongsang National University School of Medicine, Chilam-dong, Jinju 660-702, South Korea
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  • Gyu-Jin Rho,

    1. College of Veterinary Medicine, Gyeongsang National University, Gajwa-dong, Jinju 660-701, Republic of Korea
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  • Geun-Ho Maeng,

    1. College of Veterinary Medicine, Gyeongsang National University, Gajwa-dong, Jinju 660-701, Republic of Korea
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  • Ryoung-Hoon Jeon,

    1. College of Veterinary Medicine, Gyeongsang National University, Gajwa-dong, Jinju 660-701, Republic of Korea
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  • Hee-Chun Lee,

    1. College of Veterinary Medicine, Gyeongsang National University, Gajwa-dong, Jinju 660-701, Republic of Korea
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  • Jong-Ryoul Kim,

    1. Maxillofacial Center, Onhospital, Danggam-dong, Busan 614-883, South Korea
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  • Gyoo-Cheon Kim,

    1. Department of Oral anatomy, School of Dentistry, Pusan National University, Mulgeum-eup, Yangsan 626-770, South Korea
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  • June-Ho Byun

    Corresponding author
    1. Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Biomedical center (BK21), Gyeongsang National University School of Medicine, Chilam-dong, Jinju 660-702, South Korea
    • Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Biomedical center (BK21), Gyeongsang National University School of Medicine, Chilam-dong, Jinju 660-702, South Korea
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  • How to cite this article: Lee JH, Kim S-W, Kim U-K, Oh SH, June-Kim S, Park B-W, Kim J-H, Hah Y-S, Kim DR, Rho G-J, Maeng G-H, Jeon R-H, Lee H-C, Kim J-R, Kim G-C, Byun J-H.. 2013. Generation of osteogenic construct using periosteal-derived osteoblasts and polydioxanone/pluronic F127 scaffold with periosteal-derived CD146 positive endothelial-like cells. J Biomed Mater Res Part A 2013:101A:942–953.

Abstract

The purpose of this study was to generate tissue-engineered bone using human periosteal-derived osteoblasts (PO) and polydioxanone/pluronic F127 (PDO/pluronic F127) scaffold with preseeded human periosteal-derived CD146 positive endothelial-like cells (PE). PE were purified from the periosteal cell population by cell sorting. One of the important factors to consider in generating tissue-engineered bone using osteoprecursor and endothelial cells and a specific scaffold is whether the function of osteoprecursor and endothelial cells can be maintained in originally different culture medium conditions. After human PE were preseeded into PDO/pluronic F127 scaffold and cultured in endothelial cell basal medium-2 for 7 days, human PO were seeded into the PDO/pluronic F127 scaffold with PE, and then, this cell-scaffold construct was cultured in endothelial cell basal medium-2 with osteogenic induction factors, including ascorbic acid, dexamethasone, and β-glycerophosphate, for a further 7 days. Then, this 2-week cultured construct was grafted into the mandibular defect of miniature pig. Twelve weeks after implantation, the animal was sacrificed. Clinical examination revealed that newly formed bone was seen more clearly in the defect with human PO and PDO/pluronic F127 scaffold with preseeded human PE. The experimental results suggest that tissue-engineered bone formation using human PO and PDO/pluronic F127 scaffold with preseeded human PE can be used to restore skeletal integrity to various bony defects when used in clinics. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

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