How to cite this article: Chen K-Y, Dong G-C, Hsu C-Y, Chen Y-S, Yao C-H. 2013. Autologous bone marrow stromal cells loaded onto porous gelatin scaffolds containing Drynaria fortunei extract for bone repair. J Biomed Mater Res Part A 2013:101A:954–962.
Article first published online: 10 SEP 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 4, pages 954–962, April 2013
How to Cite
Chen, K.-Y., Dong, G.-C., Hsu, C.-Y., Chen, Y.-S. and Yao, C.-H. (2013), Autologous bone marrow stromal cells loaded onto porous gelatin scaffolds containing Drynaria fortunei extract for bone repair. J. Biomed. Mater. Res., 101A: 954–962. doi: 10.1002/jbm.a.34397
These authors contributed equally to this work.
- Issue published online: 21 FEB 2013
- Article first published online: 10 SEP 2012
- Manuscript Accepted: 26 JUN 2012
- Manuscript Revised: 25 MAY 2012
- Manuscript Received: 20 SEP 2011
- National Science Council of the Republic of China, Taiwan. Grant Number: NSC 98-2221-E-039-005-MY3
- China Medical University. Grant Number: CMU99-S-44
- bone marrow stromal cells;
- Drynaria fortunei;
- tricalcium phosphate
GGT-GSB composite was prepared by mixing a biodegradable GGT composite containing genipin-crosslinked gelatin and β-tricalcium phosphate with Gu-Sui-Bu extract (GSB) (Drynaria fortunei (Kunze) J. Sm.), a traditional Chinese medicine. Then, porous GGT and GGT-GSB scaffolds were fabricated using a salt-leaching method. The GGT and GGT-GSB scaffolds thus obtained had a macroporous structure and high porosity. Rabbit bone marrow stromal cells (BMSCs) were seeded onto GGT and GGT-GSB scaffolds. The biological response of rabbit calvarial bone to these scaffolds was considered to evaluate the potential of the scaffolds for use in bone tissue engineering. After 8 weeks of implantation, each scaffold induced new bone formation at a cranial bone defect, as was verified by X-ray microradiography. The BMSC-seeded GGT-GSB scaffolds induced more new bone formation than the BMSC-seeded GGT and acellular scaffolds. These observations suggest that an autologous BMSCs-seeded porous GGT-GSB scaffold can be adopted in bone engineering in vivo and has great potential for regenerating defective bone tissue. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.