How to cite this article: Meng X, Stout DA, Sun L, Beingessner RL, Fenniri H, Webster TJ. 2013. Novel injectable biomimetic hydrogels with carbon nanofibers and self assembled rosette nanotubes for myocardial applications. J Biomed Mater Res Part A 2013:101A:1095–1102.
Novel injectable biomimetic hydrogels with carbon nanofibers and self assembled rosette nanotubes for myocardial applications†
Article first published online: 24 SEP 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 4, pages 1095–1102, April 2013
How to Cite
Meng, X., Stout, D. A., Sun, L., Beingessner, R. L., Fenniri, H. and Webster, T. J. (2013), Novel injectable biomimetic hydrogels with carbon nanofibers and self assembled rosette nanotubes for myocardial applications. J. Biomed. Mater. Res., 101A: 1095–1102. doi: 10.1002/jbm.a.34400
- Issue published online: 21 FEB 2013
- Article first published online: 24 SEP 2012
- Manuscript Accepted: 25 JUL 2012
- Manuscript Revised: 24 JUL 2012
- Manuscript Received: 27 NOV 2011
- National Science Foundation Graduate Research Fellowship Program. Grant Number: NSF #1058262
- Hermann Foundation
- National Research Council of Canada
- Natural Science and Engineering Research Council of Canada
- University of Alberta
- rosette nanotubes;
- carbon nanofibers;
- poly(2-hydroxyethyl methacrylate)
The objective of the present in vitro study was to investigate cardiomyocyte functions, specifically their adhesion and proliferation, on injectable scaffolds containing RNT (rosette nanotubes) and CNF (carbon nanofibers) in a pHEMA (poly(2-hydroxyethyl methacrylate)) hydrogel to determine their potential for myocardial tissue engineering applications. RNTs are novel biocompatible nanomaterials assembled from synthetic analogs of DNA bases guanine and cytosine that self-assemble within minutes when placed in aqueous solutions at body temperatures. These materials could potentially improve cardiomyocyte functions and solidification time of pHEMA and CNF composites. Because heart tissue is conductive, CNFs were added to pHEMA to increase the composite's conductivity. Our results showed that cardiomyocyte density increased after 4 h, 1 day, and 3 days with greater amounts of CNFs and greater amounts of RNTs in pHEMA (up to 10 mg mL−1 CNFs and 0.05 mg mL−1 RNTs). Factors that may have increased cardiomyocyte functions include greater wettability, conductivity, and an increase in surface nanoroughness with greater amounts of CNFs and RNTs. In effect, contact angles measured on the surface of the composites decreased while the conductivity and surface roughness increased as CNFs and RNTs content increased. Lastly, the ultimate tensile modulus decreased for composites with greater amounts of CNFs. In summary, the properties of these injectable composites make them promising candidates for myocardial tissue engineering applications and should be further studied. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.