How to cite this article: Yañez-Soto B, Liliensiek SJ, Murphy CJ, Nealey PF. 2013. Biochemically and topographically engineered poly(ethylene glycol) diacrylate hydrogels with biomimetic characteristics as substrates for human corneal epithelial cells. J Biomed Mater Res Part A 2013:101A:1184–1194.
Biochemically and topographically engineered poly(ethylene glycol) diacrylate hydrogels with biomimetic characteristics as substrates for human corneal epithelial cells†
Article first published online: 18 DEC 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 4, pages 1184–1194, April 2013
How to Cite
Yañez-Soto, B., Liliensiek, S. J., Murphy, C. J. and Nealey, P. F. (2013), Biochemically and topographically engineered poly(ethylene glycol) diacrylate hydrogels with biomimetic characteristics as substrates for human corneal epithelial cells. J. Biomed. Mater. Res., 101A: 1184–1194. doi: 10.1002/jbm.a.34412
- Issue published online: 21 FEB 2013
- Article first published online: 18 DEC 2012
- Manuscript Accepted: 6 AUG 2012
- Manuscript Revised: 18 JUN 2012
- Manuscript Received: 13 MAR 2012
- NIH-National Eye Institute. Grant Number: 1RO1EY017367-01A and 1RO1EY016134-01A2
- NIH-National Institute of Arthritis and Musculoskeletal and Skin Diseases. Grant Number: 5RC2AR058971-01
- biomimetic material;
- epithelial cell;
- polyethylene glycol;
Incorporation of biophysical and biochemical cues into the design of biomaterials is an important strategy for tissue engineering, the design of biomedical implants and cell culture. Hydrogels synthesized from poly(ethylene glycol) diacrylate (PEGDA) were investigated as a platform to simultaneously present human corneal epithelial cells (HCECs) in vitro with topography and adhesion peptides to mimic the native physical and chemical attributes of the basement membrane underlying the epithelium in vivo. Hydrogels synthesized from aqueous solutions of 20% PEGDA (Mw = 3400 g/mol) prevented nonspecific cell adhesion and were functionalized with the integrin-binding peptide Arg-Gly-Asp (RGD) in concentrations from 5 to 20 mM. The hydrogels swelled minimally after curing and were molded with ridge and groove features with lateral dimensions from 200 to 2000 nm and 300-nm depth. HCECs were cultured on topographic surfaces functionalized with RGD and compared with control unfunctionalized topographic substrates. HCEC alignment, either parallel or perpendicular to ridges, was influenced by the culture media on substrates promoting nonspecific attachment. In contrast, the alignment of HCECs cultured on RGD hydrogels showed substantially less dependence on the culture media. In the latter case, the moldable RGD-functionalized hydrogels allowed for decoupling the cues from surface chemistry, soluble factors, and topography that simultaneously impact HCEC behavior. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.