How to cite this article: Raghunathan VK, McKee CT, Tocce EJ, Nealey PF, Russell P, Murphy CJ. 2013. Nuclear and cellular alignment of primary corneal epithelial cells on topography. J Biomed Mater Res Part A 2013:101A:1069–'1079.
Nuclear and cellular alignment of primary corneal epithelial cells on topography†
Version of Record online: 11 SEP 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 4, pages 1069–1079, April 2013
How to Cite
Raghunathan, V. K., McKee, C. T., Tocce, E. J., Nealey, P. F., Russell, P. and Murphy, C. J. (2013), Nuclear and cellular alignment of primary corneal epithelial cells on topography. J. Biomed. Mater. Res., 101A: 1069–1079. doi: 10.1002/jbm.a.34417
- Issue online: 21 FEB 2013
- Version of Record online: 11 SEP 2012
- Manuscript Accepted: 6 AUG 2012
- Manuscript Revised: 3 JUL 2012
- Manuscript Received: 15 MAY 2012
- National Institute of Health through grants from the National Eye Institute. Grant Numbers: R01EY01613404, P30EY12576
- Research to Prevent Blindness
- epithelial cells;
- contact guidance;
- biophysical cues;
The basement membrane of the corneal epithelium presents biophysical cues in the form of topography and compliance that can modulate cytoskeletal dynamics, which, in turn, can result in altering cellular and nuclear morphology and alignment. In this study, the effect of topographic patterns of alternating ridges and grooves on nuclear and cellular shape and alignment was determined. Primary corneal epithelial cells were cultured on either planar or topographically patterned (400–4000 nm pitch) substrates. Alignment of individual cell body was correlated with respective nucleus for the analysis of orientation and elongation. A biphasic response in alignment was observed. Cell bodies preferentially aligned perpendicular to the 800 nm pitch; and with increasing pitch, cells increasingly aligned parallel to the substratum. Nuclear orientation largely followed this trend with the exception of those on 400 nm. On this biomimetic size scale, some nuclei oriented perpendicular to the topography while their cytoskeleton elements aligned parallel. Both nuclei and cell bodies were elongated on topography compared to those on flat surfaces. Our data demonstrate that nuclear orientation and shape are differentially altered by topographic features that are not mandated by alignment of the cell body. This novel finding suggests that nuanced differences in alignment of the nucleus versus the cell body exist and that these differences could have consequences on gene and protein regulation that ultimately regulate cell behaviors. A full understanding of these mechanisms could disclose novel pathways that would better inform evolving strategies in cell, stem cell, and tissue engineering as well as the design and fabrication of improved prosthetic devices. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.