How to cite this article: Ma Y, Zhang Y, Liu Y, Chen L, Li S, Zhao W, Sun G, Li N, Wang Y, Guo X, Lv G, Ma X. 2013. Investigation of alginate-ϵ-poly-L-lysine microcapsules for cell microencapsulation. J Biomed Mater Res Part A 2013:101A:1265–1273.
Investigation of alginate–ϵ-poly-L-lysine microcapsules for cell microencapsulation†
Article first published online: 15 OCT 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 5, pages 1265–1273, May 2013
How to Cite
Ma, Y., Zhang, Y., Liu, Y., Chen, L., Li, S., Zhao, W., Sun, G., Li, N., Wang, Y., Guo, X., Lv, G. and Ma, X. (2013), Investigation of alginate–ϵ-poly-L-lysine microcapsules for cell microencapsulation. J. Biomed. Mater. Res., 101A: 1265–1273. doi: 10.1002/jbm.a.34418
- Issue published online: 25 MAR 2013
- Article first published online: 15 OCT 2012
- Manuscript Accepted: 30 JUL 2012
- Manuscript Revised: 12 JUL 2012
- Manuscript Received: 26 MAR 2012
- National Natural Science Foundation of China. Grant Numbers: 20906088, 21076207, 10979050, 20806080, 30970885, 81101757, 81171550, 51103157
- Natural Science Foundation of Liaoning Province. Grant Number: 20101087
- Science and technology fund of Dalian. Grant Number: 2008J23JH023
- National Key Sci-Tech Special Project of China. Grant Number: 2008ZX10002-019
- Strategic Priority Research Program of the Chinese Academy of Sciences. Grant Number: XDA01030303
- poly-amino acid membrane;
- cell culture
Cell microencapsulation is a promising approach for cell implantation, cell-based gene therapy, and large-scale cell culture. The well-studied α-AP (alginate–α-poly-L-lysine) microcapsules have been restricted to large-scale cell-culture and clinical applications because of high costs and cytotoxic effects in some cases. This study used ϵ-poly-L-lysine (ϵ-PLL), a high-biocompatible and low-cost food additives produced by fermentation, to prepare ϵ-AP (alginate–ϵ-PLL) microcapsules with various thickness membranes and swelling behaviors. ϵ-AP microcapsules were permeable to BSA, a standard protein of culture medium. ϵ-AP-microencapsulated Chinese hamster ovary (CHO) cells proliferated with culture time; no obvious difference with α-AP-microencapsulated CHO cells during the early 19 days. Whereas ϵ-AP-microencapsulated CHO cells kept higher viability (OD = 0.646 ± 0.012) on the 22nd day and microcapsule strength (integrity rate of 88%) on the 24th day than that of α-AP microcapsules (OD = 0.558 ± 0.025, integrity rate of 80%). ϵ-AP (alginate-ϵ-PLL) microcapsules exhibited more superior properties and could lower the costs to broaden the applications of microencapsulation technology. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.