Get access

Water-stable electrospun collagen fibers from a non-toxic solvent and crosslinking system

Authors

  • Qiuran Jiang,

    1. Department of Textiles, Merchandising & Fashion Design, 234, HECO Building, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0802
    Search for more papers by this author
  • Narendra Reddy,

    1. Department of Textiles, Merchandising & Fashion Design, 234, HECO Building, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0802
    Search for more papers by this author
  • Simeng Zhang,

    1. Department of Biological Systems Engineering, 234, HECO Building, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0802
    Search for more papers by this author
  • Nicholas Roscioli,

    1. Kensey Nash Corporation, 735 Pennsylvania Drive, Exton, Pennsylvania 19341
    Search for more papers by this author
  • Yiqi Yang

    Corresponding author
    1. Department of Textiles, Merchandising & Fashion Design, 234, HECO Building, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0802
    2. Department of Biological Systems Engineering, 234, HECO Building, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0802
    3. Nebraska Center for Materials and Nanoscience, 234, HECO Building, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0802
    • Department of Textiles, Merchandising & Fashion Design, 234, HECO Building, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0802
    Search for more papers by this author

  • How to cite this article: Jiang Q, Reddy N, Zhang S, Roscioli N, Yang Y. 2013. Water-stable electrospun collagen fibers from a nontoxic solvent and crosslinking system. J Biomed Mater Res Part A 2013:101A:1237–1247.

Abstract

Cytocompatible and water-stable ultrafine collagen fibers were electrospun by dissolving collagen in a low corrosive ethanol–water solvent and crosslinked by citric acid (CA) with glycerol as the crosslinking extender. Conventional solvents used for electrospinning of collagen either cause denaturation or contain more than 50% salt potentially leading to poor mechanical properties and water stability of the scaffolds. Collagen scaffolds have to be modified by techniques, such as, crosslinking to overcome the limitations in strength and stability. However, the existing crosslinking methods are either cytotoxic or ineffective. In this research, a benign ethanol-water solvent system and an extender-aided CA crosslinking method were developed. The native collagen conformation was retained after electrospinning, and the dry/wet strengths and water stability of fibers were substantially enhanced after crosslinking. The crosslinked electrospun scaffolds could maintain their fibrous structure for up to 30 days in phosphate-buffered saline at 37°C. Cells exhibited better attachment and growth on the CA crosslinked collagen fibers than on the glutaraldehyde crosslinked scaffolds. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

Ancillary