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Time-dependent release of growth factors from implant surfaces treated with plasma rich in growth factors

Authors

  • María Belén Sánchez-Ilárduya,

    1. Biosurfaces Unit, CIC biomaGUNE, Paseo Miramón 182, 20009 San Sebastián, Spain
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  • Elodie Trouche,

    1. Biosurfaces Unit, CIC biomaGUNE, Paseo Miramón 182, 20009 San Sebastián, Spain
    2. BTI I+D, José Ma Cagigal 19, 01007 Vitoria, Spain
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  • Ricardo Tejero,

    1. Biosurfaces Unit, CIC biomaGUNE, Paseo Miramón 182, 20009 San Sebastián, Spain
    2. BTI I+D, José Ma Cagigal 19, 01007 Vitoria, Spain
    3. Department of Biochemistry and Molecular Biology, University of the Basque Country, 48940 Leioa, Spain
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  • Gorka Orive,

    1. BTI I+D, José Ma Cagigal 19, 01007 Vitoria, Spain
    2. NanoBioCel Group, Laboratory of Pharmaceutics, University of the Basque Country, School of Pharmacy, Vitoria-Gasteiz, Spain. Networking Biomedical Research Center on Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, Vitoria-Gasteiz, Spain
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  • Ilya Reviakine,

    Corresponding author
    1. Biosurfaces Unit, CIC biomaGUNE, Paseo Miramón 182, 20009 San Sebastián, Spain
    2. Department of Biochemistry and Molecular Biology, University of the Basque Country, 48940 Leioa, Spain
    • Biosurfaces Unit, CIC biomaGUNE, Paseo Miramón 182, 20009 San Sebastián, Spain
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  • Eduardo Anitua

    Corresponding author
    1. BTI I+D, José Ma Cagigal 19, 01007 Vitoria, Spain
    2. Private Practice in Implantology and Oral Rehabilitation, José Ma Cagigal 19, 01007 Vitoria, Spain
    • BTI I+D, José Ma Cagigal 19, 01007 Vitoria, Spain
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  • This article was published online on 7 November 2012. An error was subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected on 22 January 2013.

  • How to cite this article: Sánchez-Ilárduya MB, Trouche E, Tejero R, Orive G, Reviakine I, Anitua E. 2013. Time-dependent release of growth factors from implant surfaces treated with plasma rich in growth factors. J Biomed Mater Res Part A 2013:101A:1478–1488.

Abstract

Plasma rich in growth factors (PRGFs) technology is an autologous platelet-rich plasma approach that provides a pool of growth factors and cytokines that have been shown to increase tissue regeneration and accelerate dental implant osseointegration. In this framework, the spatiotemporal release of growth factors and the establishment of a provisional fibrin matrix are likely to be key aspects governing the stimulation of the early phases of tissue regeneration around implants. We investigated the kinetics of growth factor release at implant surfaces functionalized either with PRGFs or platelet-poor plasma and correlated the results obtained with the morphology of the resulting interfaces. Our main finding is that activation and clot formation favors longer residence times of the growth factors at the interfaces studied, probably due to their retention in the adsorbed fibrin matrix. The concentration of the platelet-derived growth factors above the interfaces becomes negligible after 2–4 days and is significantly higher in the case of activated interfaces than in the case of nonactivated ones, whereas that of the plasmatic hepatocyte growth factor is independent of platelet concentration and activation, and remains significant for up to 9 days. Platelet-rich plasma preparations should be activated to permit growth factor release and thereby facilitate implant surface osseointegration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

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