How to cite this article: Yin A, Zhang K, McClure MJ, Huang C, Wu J, Fang J, Mo X, Bowlin GL, Al-Deyab SS, El-Newehy M. 2013. Electrospinning collagen/chitosan/poly(L-lactic acid-co-ϵ-caprolactone) to form a vascular graft: Mechanical and biological characterization. J Biomed Mater Res Part A 2013:101A:1292–1301.
Electrospinning collagen/chitosan/poly(L-lactic acid-co-ϵ-caprolactone) to form a vascular graft: Mechanical and biological characterization†
Article first published online: 15 OCT 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 5, pages 1292–1301, May 2013
How to Cite
Yin, A., Zhang, K., McClure, M. J., Huang, C., Wu, J., Fang, J., Mo, X., Bowlin, G. L., Al-Deyab, S. S. and El-Newehy, M. (2013), Electrospinning collagen/chitosan/poly(L-lactic acid-co-ϵ-caprolactone) to form a vascular graft: Mechanical and biological characterization. J. Biomed. Mater. Res., 101A: 1292–1301. doi: 10.1002/jbm.a.34434
- Issue published online: 25 MAR 2013
- Article first published online: 15 OCT 2012
- Manuscript Accepted: 28 AUG 2012
- Manuscript Revised: 24 AUG 2012
- Manuscript Received: 11 JUN 2012
- National High Technology Research and Development Program. Grant Number: 2008AA03Z305
- Science and Technology Commission of Shanghai Municipality Program. Grant Number: 11nm0506200
- National Nature Science Foundation of China. Grant Number: 31070871
- The National Plan for Science and Technology. Grant Number: 10-NAN1013-02
- Doctorial Innovation Fund of Donghua University. Grant Number: BC201127
- collagen/chitosan/P(LLA-CL) blend;
- mechanical properties;
- vascular graft
For blood vessel tissue engineering, an ideal vascular graft should possess excellent biocompatibility and mechanical properties. For this study, a elastic material of poly (L-lactic acid-co-ϵ-caprolactone) (P(LLA-CL)), collagen and chitosan blended scaffold at different ratios were fabricated by electrospinning. Upon fabrication, the scaffolds were evaluated to determine the tensile strength, burst pressure, and dynamic compliance. In addition, the contact angle and endothelial cell proliferation on the scaffolds were evaluated to demonstrate the structures potential to serve as a vascular prosthetic capable of in situ regeneration. The collagen/chitosan/P(LLA-CL) scaffold with the ratio of 20:5:75 reached the highest tensile strength with the value of 16.9 MPa, and it was elastic with strain at break values of ∼112%, elastic modulus of 10.3 MPa. The burst pressure strength of the scaffold was greater than 3365 mmHg and compliance value was 0.7%/100 mmHg. Endothelial cells proliferation was significantly increased on the blended scaffolds versus the P(LLA-CL). Meanwhile, the endothelial cells were more adherent based on the increase in the degree of cell spreading on the surface of collagen/chitosan/P(LLA-CL) scaffolds. Such blended scaffold especially with the ratio of 20:5:75 thus has the potential for vascular graft applications. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.