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Electrospinning collagen/chitosan/poly(L-lactic acid-co-ϵ-caprolactone) to form a vascular graft: Mechanical and biological characterization

Authors

  • Anlin Yin,

    1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China
    2. Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia 23284-3067
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  • Kuihua Zhang,

    1. Department of Textile Engineering, Jiaxing University, Zhejiang 314001, China
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  • Michael J. McClure,

    1. Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia 23284-3067
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  • Chen Huang,

    1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China
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  • Jinglei Wu,

    1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China
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  • Jun Fang,

    1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China
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  • Xiumei Mo,

    Corresponding author
    1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China
    • State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China
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  • Gary L. Bowlin,

    Corresponding author
    1. Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia 23284-3067
    • Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia 23284-3067
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  • Salem S. Al-Deyab,

    1. Petrochemical Research Chair, Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
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  • Mohamed El-Newehy

    1. Petrochemical Research Chair, Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
    2. Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt
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  • How to cite this article: Yin A, Zhang K, McClure MJ, Huang C, Wu J, Fang J, Mo X, Bowlin GL, Al-Deyab SS, El-Newehy M. 2013. Electrospinning collagen/chitosan/poly(L-lactic acid-co-ϵ-caprolactone) to form a vascular graft: Mechanical and biological characterization. J Biomed Mater Res Part A 2013:101A:1292–1301.

Abstract

For blood vessel tissue engineering, an ideal vascular graft should possess excellent biocompatibility and mechanical properties. For this study, a elastic material of poly (L-lactic acid-co-ϵ-caprolactone) (P(LLA-CL)), collagen and chitosan blended scaffold at different ratios were fabricated by electrospinning. Upon fabrication, the scaffolds were evaluated to determine the tensile strength, burst pressure, and dynamic compliance. In addition, the contact angle and endothelial cell proliferation on the scaffolds were evaluated to demonstrate the structures potential to serve as a vascular prosthetic capable of in situ regeneration. The collagen/chitosan/P(LLA-CL) scaffold with the ratio of 20:5:75 reached the highest tensile strength with the value of 16.9 MPa, and it was elastic with strain at break values of ∼112%, elastic modulus of 10.3 MPa. The burst pressure strength of the scaffold was greater than 3365 mmHg and compliance value was 0.7%/100 mmHg. Endothelial cells proliferation was significantly increased on the blended scaffolds versus the P(LLA-CL). Meanwhile, the endothelial cells were more adherent based on the increase in the degree of cell spreading on the surface of collagen/chitosan/P(LLA-CL) scaffolds. Such blended scaffold especially with the ratio of 20:5:75 thus has the potential for vascular graft applications. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

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