How to cite this article: Haroun AA, Abo-Zeid MA, Youssef AM, Gamal-Eldeen A. 2013. In vitro biological study of gelatin/PLG nanocomposite using MCF-7 breast cancer cells. J Biomed Mater Res Part A 2013:101A:1388–1396.
In vitro biological study of gelatin/PLG nanocomposite using MCF-7 breast cancer cells †
Article first published online: 18 OCT 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 5, pages 1388–1396, May 2013
How to Cite
Haroun, A. A., Abo-Zeid, M. A., Youssef, A. M. and Gamal-Eldeen, A. (2013), In vitro biological study of gelatin/PLG nanocomposite using MCF-7 breast cancer cells . J. Biomed. Mater. Res., 101A: 1388–1396. doi: 10.1002/jbm.a.34441
- Issue published online: 25 MAR 2013
- Article first published online: 18 OCT 2012
- Manuscript Accepted: 28 AUG 2012
- Manuscript Revised: 25 AUG 2012
- Manuscript Received: 25 DEC 2011
- National Research Center, Cairo, Egypt. Grant Number: 9100401/2010-2013
- MCF-7 cells;
- poly (DL-lactide-co-glycolide);
- TiO2 nanowires
This work aimed to evaluate new materials to be carried out as scaffolds using breast cancer cells MCF-7. These new nanocomposites were prepared through blending of gelatin with poly (DL-lactide-co-glycolide) (PLG) in presence of titanium nanowires (TiO2) and cartilage powder (CP). The prepared nanomaterials were characterized using Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscope, and transmitting electron microscope. Moreover, the MCF-7 cells were in vitro tested with apoptosis/necrosis assay, micronucleus test, and DNA fragmentation and MMT assay. TiO2 nanowires and CP particles have diameters around 28–128 and 17–20 nm, respectively. These were coated with gelatin matrix. Seeding of MCF-7 cells with the prepared nanomaterials revealed high cell attachment to their surfaces and they were viable after 72 h. It has been shown that the prepared nanocomposites did not induce necrotic effects on MCF-7 cells; however, they induced a significant DNA fragmentation in comparison with the nontreated control cells. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.