Mineralized poly(lactic acid) scaffolds loading vascular endothelial growth factor and the in vivo performance in rat subcutaneous model

Authors

  • Joong-Hyun Kim,

    1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, South Korea
    2. Biomaterials and Tissue Engineering Laboratory, Department of Nanobiomedical Science and WCU Research Center, Dankook University, South Korea
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  • Tae-Hyun Kim,

    1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, South Korea
    2. Biomaterials and Tissue Engineering Laboratory, Department of Nanobiomedical Science and WCU Research Center, Dankook University, South Korea
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  • Guang-Zhen Jin,

    1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, South Korea
    2. Biomaterials and Tissue Engineering Laboratory, Department of Nanobiomedical Science and WCU Research Center, Dankook University, South Korea
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  • Jeong-Hui Park,

    1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, South Korea
    2. Biomaterials and Tissue Engineering Laboratory, Department of Nanobiomedical Science and WCU Research Center, Dankook University, South Korea
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  • Ye-Rang Yun,

    1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, South Korea
    2. Biomaterials and Tissue Engineering Laboratory, Department of Nanobiomedical Science and WCU Research Center, Dankook University, South Korea
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  • Jun-Hyeog Jang,

    1. Department of Biochemistry, Inha University College of Medicine, South Korea
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  • Hae-Won Kim

    Corresponding author
    1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, South Korea
    2. Biomaterials and Tissue Engineering Laboratory, Department of Nanobiomedical Science and WCU Research Center, Dankook University, South Korea
    3. Department of Biomaterials Science, School of Dentistry, Dankook University, South Korea
    • Institute of Tissue Regeneration Engineering (ITREN), Dankook University, South Korea
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  • How to cite this article: Kim J-H, Kim T-H, Jin G-Z, Park J-H, Yun Y-R, Jang J-H, Kim H-W. 2013. Mineralized poly(lactic acid) scaffolds loading vascular endothelial growth factor and the in vivo performance in rat subcutaneous model. J Biomed Mater Res Part A 2013:101A:1447–1455.

Abstract

The functionalization of degradable polymeric scaffolds with therapeutic molecules such as vascular endothelial growth factor (VEGF) is a key strategy to gain better regenerative ability of damaged bone tissue by stimulating vascularization and tissue perfusion. Here, we combined VEGF with poly(lactic acid) (PLA) porous scaffold, after modifying the PLA surface with calcium phosphate (CaP) mineral. The mineralized PLA scaffold (mPLA) showed more effective loading capacity of VEGF than the PLA without mineralization as well as profiled sustainable release of VEGF for up to a couple of weeks. The VEGF-loaded mPLA scaffold presented significantly improved proliferation of primary endothelial cells for up to 7 days, with respect to the scaffold without the VEGF loading. The performance of the engineered scaffold was assessed after subcutaneous implantation in rats for 4 weeks. Histological results showed favorable tissue compatibility of both the mPLA scaffolds (with and without VEGF loading), as characterized by infiltration of inflammatory cells, formation of fibrous capsule, and ingrowth of fibroblasts into the matrices. Immunohistochemical staining of the von Willebrand Factor revealed significantly improved formation of neo-capillaries in the VEGF-loaded mPLA. Based on this study, the strategy of VEGF loading onto mineralized PLA scaffold is considered beneficial for gaining improved vascularization of the polymeric scaffolds, suggesting potential applications for bone tissue engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

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