This work was supported by the Priority Research Centers Program (no. 2009-0093829) and WCU Program (no. R31-10069)
Article first published online: 27 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 6, pages 1651–1660, June 2013
How to Cite
Kim, T.-H., Kim, M., Eltohamy, M., Yun, Y.-R., Jang, J.-H. and Kim, H.-W. (2013), Efficacy of mesoporous silica nanoparticles in delivering BMP-2 plasmid DNA for in vitro osteogenic stimulation of mesenchymal stem cells. J. Biomed. Mater. Res., 101A: 1651–1660. doi: 10.1002/jbm.a.34466
How to cite this article: Kim T-H, Kim M, Eltohamy M, Yun Y-R, Jang J-H, Kim H-W. 2013. Efficacy of mesoporous silica nanoparticles in delivering BMP-2 plasmid DNA for in vitro osteogenic stimulation of mesenchymal stem cells. J Biomed Mater Res Part A 2013:101A:1651–1660.
- Issue published online: 13 APR 2013
- Article first published online: 27 NOV 2012
- Manuscript Accepted: 24 SEP 2012
- Manuscript Revised: 2 SEP 2012
- Manuscript Received: 24 JUN 2012
- National Research Foundation
- Ministry of Education, Science and Technology, South Korea
- bone morphogenetic protein;
- mesoporous silica nanoparticles;
- gene delivery;
- mesenchymal stem cells;
- bone regeneration
We report the ability of aminated mesoporous silica nanoparticles (MSN–NH2) with large mesopore space and positive-charged surface to deliver genes within rat mesenchymal stem cells (MSCs). The amine functionalized inorganic nanoparticles were complexed with bone morphogenetic protein-2 (BMP2) plasmid DNA (pDNA) to study their transfection efficiency in MSCs. Intracellular uptake of the complex BMP2 pDNA/MSN–NH2 occurred significantly, with a transfection efficiency of approximately 68%. Furthermore, over 66% of the transfected cells produced BMP2 protein. The osteogenic differentiation of the transfected MSCs was demonstrated by the expression of bone-related genes and proteins including bone sialoprotein, osteopontin, and osteocalcin. The MSN–NH2 delivery vehicle for BMP2 pDNA developed in this study may be a potential gene delivery system for bone tissue regeneration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.