How to cite this article: Nie FL, Zheng YF, Wei SC, Wang DS, Yu ZT, Salimgareeva GK, Polyakov AV, Valiev RZ. 2013. In vitro and in vivo studies on nanocrystalline Ti fabricated by equal channel angular pressing with microcrystalline CP Ti as control. J Biomed Mater Res Part A 2013:101A:1694–1707.
In vitro and in vivo studies on nanocrystalline Ti fabricated by equal channel angular pressing with microcrystalline CP Ti as control†
Article first published online: 27 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 6, pages 1694–1707, June 2013
How to Cite
Nie, F. L., Zheng, Y. F., Wei, S.C., Wang, D. S., Yu, Z. T., Salimgareeva, G. K., Polyakov, A. V. and Valiev, R. Z. (2013), In vitro and in vivo studies on nanocrystalline Ti fabricated by equal channel angular pressing with microcrystalline CP Ti as control. J. Biomed. Mater. Res., 101A: 1694–1707. doi: 10.1002/jbm.a.34472
- Issue published online: 13 APR 2013
- Article first published online: 27 NOV 2012
- Manuscript Accepted: 1 OCT 2012
- Manuscript Revised: 19 SEP 2012
- Manuscript Received: 6 JUL 2012
- National Basic Research Program of China (973 Program). Grant Numbers: 2007CB936103, 2012CB619102
- National High Technology Research and Development Program of China (863 Program). Grant Numbers: 2011AA030101, 2011AA030103
- National Natural Science Foundation of China. Grant Number: 31170909
- bulk nanocrystalline Ti;
- equal channel angular pressing;
- bone growing;
Bulk nanocrystalline Ti bars (Grade 4, ϕ4 × 3000 mm3) were massively fabricated by equal channel angular pressing (ECAP) via follow-up conform scheme with the microcrystalline CP Ti as raw material. Homogeneous nanostructured crystals with the average grain size of 250 nm were identified for the ECAPed Ti, with extremely high tensile/fatigue strength (around 1240/620 MPa) and adorable elongation (more than 5%). Pronounced formation of bonelike apatite for the nanocrystalline Ti group after 14 days static immersion in simulated body fluids (SBF) reveals the prospective in vitro bioactive capability of fast calcification, whereas an estimated 17% increment in protein adsorption represents good bioaffinity of nanocrystalline Ti. The documentation onto the whole life circle of osteoblast cell lines (MG63) revealed the strong interactions and superior cellular functionalization when they are co-incubated with bulk nanocrystalline Ti sample. Moreover, thread-structured specimens were designed and implanted into the tibia of Beagles dogs till 12 weeks to study the in vivo responses between bone and metallic implant made of bulk nanocrystalline Ti, with the microcrystalline Ti as control. For the implanted nanostructured Ti group, neoformed bone around the implants underwent the whole-stage transformation proceeding from originally osteons or immature woven bone to mature lamellar bone (skeletonic trabecular), even with the remodeling being finished till 12 weeks. The phenomenal osseointegration of direct implant-bone contact can be revealed from the group of the ECAPed Ti without fibrous tissue encapsulation in the gap between the implant and autogenous bone. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.