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In vitro and in vivo studies on nanocrystalline Ti fabricated by equal channel angular pressing with microcrystalline CP Ti as control

Authors

  • F. L. Nie,

    1. State Key Laboratory for Turbulence and Complex System and Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China
    2. Center for Medical Device Evaluation, SFDA, Beijing 100044, China
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  • Y. F. Zheng,

    Corresponding author
    1. State Key Laboratory for Turbulence and Complex System and Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China
    2. Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
    • State Key Laboratory for Turbulence and Complex System and Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China
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  • S.C. Wei,

    Corresponding author
    1. Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
    2. Center for Biomaterials and Medical Devices, School of Stomatology, Peking University, Beijing 100081, China
    • Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
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  • D. S. Wang,

    1. Institute of Stomatology, Chinese PLA General Hospital, Beijing 100853, China
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  • Z. T. Yu,

    1. Biomaterials Research Center, Northwest Institute for Nonferrous Metal Research, Xian 710016, China
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  • G. K. Salimgareeva,

    1. Institute of Physics of Advanced Materials, Ufa State Aviation Technical University, Ufa, Russia
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  • A. V. Polyakov,

    1. Institute of Physics of Advanced Materials, Ufa State Aviation Technical University, Ufa, Russia
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  • R. Z. Valiev

    Corresponding author
    1. Institute of Physics of Advanced Materials, Ufa State Aviation Technical University, Ufa, Russia
    • Institute of Physics of Advanced Materials, Ufa State Aviation Technical University, Ufa, Russia
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  • How to cite this article: Nie FL, Zheng YF, Wei SC, Wang DS, Yu ZT, Salimgareeva GK, Polyakov AV, Valiev RZ. 2013. In vitro and in vivo studies on nanocrystalline Ti fabricated by equal channel angular pressing with microcrystalline CP Ti as control. J Biomed Mater Res Part A 2013:101A:1694–1707.

Abstract

Bulk nanocrystalline Ti bars (Grade 4, ϕ4 × 3000 mm3) were massively fabricated by equal channel angular pressing (ECAP) via follow-up conform scheme with the microcrystalline CP Ti as raw material. Homogeneous nanostructured crystals with the average grain size of 250 nm were identified for the ECAPed Ti, with extremely high tensile/fatigue strength (around 1240/620 MPa) and adorable elongation (more than 5%). Pronounced formation of bonelike apatite for the nanocrystalline Ti group after 14 days static immersion in simulated body fluids (SBF) reveals the prospective in vitro bioactive capability of fast calcification, whereas an estimated 17% increment in protein adsorption represents good bioaffinity of nanocrystalline Ti. The documentation onto the whole life circle of osteoblast cell lines (MG63) revealed the strong interactions and superior cellular functionalization when they are co-incubated with bulk nanocrystalline Ti sample. Moreover, thread-structured specimens were designed and implanted into the tibia of Beagles dogs till 12 weeks to study the in vivo responses between bone and metallic implant made of bulk nanocrystalline Ti, with the microcrystalline Ti as control. For the implanted nanostructured Ti group, neoformed bone around the implants underwent the whole-stage transformation proceeding from originally osteons or immature woven bone to mature lamellar bone (skeletonic trabecular), even with the remodeling being finished till 12 weeks. The phenomenal osseointegration of direct implant-bone contact can be revealed from the group of the ECAPed Ti without fibrous tissue encapsulation in the gap between the implant and autogenous bone. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

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