How to cite this article: Kwok CB, Ho FC, Li CW, Ngan A. H. W., Chan D., Chan BP. 2013. Compression-induced alignment and elongation of human mesenchymal stem cell (hMSC) in 3D collagen constructs is collagen concentration dependent. J Biomed Mater Res Part A 2013:101A:1716–1725.
Compression-induced alignment and elongation of human mesenchymal stem cell (hMSC) in 3D collagen constructs is collagen concentration dependent†
Version of Record online: 27 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 6, pages 1716–1725, June 2013
How to Cite
Kwok, C. B., Ho, F. C., Li, C. W., Ngan, A. H. W., Chan, D. and Chan, B. P. (2013), Compression-induced alignment and elongation of human mesenchymal stem cell (hMSC) in 3D collagen constructs is collagen concentration dependent. J. Biomed. Mater. Res., 101A: 1716–1725. doi: 10.1002/jbm.a.34475
- Issue online: 13 APR 2013
- Version of Record online: 27 NOV 2012
- Manuscript Accepted: 24 SEP 2012
- Manuscript Revised: 16 AUG 2012
- Manuscript Received: 29 MAR 2012
- AOSpine. Grant Number: AOSBRC-07-06
- Innovation and Technology Commission, the Hong Kong Government. Grant Number: ITS-552-09FP
- Seed Funding for Basic Science of the University of Hong Kong. Grant Number: 201011159030
- Strategic Research Themes of the University of Hong Kong on Biomedical Engineering and Bionanotechnology
- mesenchymal stem cell;
Controlling cell organization is important in tissue engineering. Guidance by aligned features on scaffolds or stimulation by physical signals can be used to induce cell alignment. We have previously demonstrated a preferred alignment of human MSCs (hMSCs) along the compression loading axis in 3D collagen construct. In this study, we aim to investigate the collagen concentration dependence of the compression-induced hMSC organization. Results demonstrated that the compression-induced alignment and elongation of hMSCs exhibited a biphasic dose-dependent relationship with collagen concentration, and associated well with both collagen ligand density and elastic modulus of the constructs. Moreover, collagen concentration and compression loading significantly affected the expression level of integrin beta 1 and antibody neutralization against this molecule aborted the compression-induced alignment and elongation responses. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.