How to cite this article: Akbari M, Dadadashian F, Kordestani SS, Xue M, Jackson CJ. 2013. Enzymatic modification of regenerated cellulosic fabrics to improve bacteria sorption properties. J Biomed Mater Res Part A 2013:101A:1734–1742.
Enzymatic modification of regenerated cellulosic fabrics to improve bacteria sorption properties†
Article first published online: 27 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 6, pages 1734–1742, June 2013
How to Cite
Akbari, M., Dadadashian, F., Kordestani, S. S., Xue, M. and Jackson, C. J. (2013), Enzymatic modification of regenerated cellulosic fabrics to improve bacteria sorption properties. J. Biomed. Mater. Res., 101A: 1734–1742. doi: 10.1002/jbm.a.34478
- Issue published online: 13 APR 2013
- Article first published online: 27 NOV 2012
- Manuscript Accepted: 4 OCT 2012
- Manuscript Revised: 26 SEP 2012
- Manuscript Received: 27 AUG 2012
- cellulase enzyme;
- bacteria sorption;
- biomedical applications
This research investigates the effect of enzymatic treatment of two different regenerated cellulosic fibers (Lyocell and viscose) on their ability of bacteria sorption from an aqueous suspension. The sorption of Escherichia coli (E. coli, Gram negative) and Staphylococcus aureus (S. aureus, Gram positive) cells by treated Lyocell and viscose fabrics were determined by measuring the optical density (OD) of the remaining bacteria suspension after removal of the fabric samples using spectrometry. Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) were utilized to investigate structural and morphological changes of the enzyme treated samples. The result showed that the moisture content and crystallinity of both viscose and Lyocell samples increased after enzymatic treatment. Comparing the results of OD measurements indicated that enzymatic treatment of cellulosic samples significantly increased the bacteria absorption properties compared to those untreated samples. However, treated samples showed different ranges of sorption ability with different kinds of bacteria. The maximum bacteria sorption of 38% and 37% of E. coli bacteria from an aqueous suspension was found for the treated viscose and Lyocell samples compared with only 20% and 10% of the untreated viscose and Lyocell samples, respectively. It was also found that S. aureus sorption of cellulose-treated viscose and Lyocell fabrics from a bacterial suspension could significantly improve up to 33% compared with only 5% of untreated samples. Furthermore, SEM micrographs confirmed that bacterial sorption of the cellulose-treated samples were effectively improved in terms of their uniform sorption on the fibers surface. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.