How to cite this article: Singarapu K, Pal I, Ramsey JD. 2013. Polyethylene glycol-grafted polyethylenimine used to enhance adenovirus gene delivery. J Biomed Mater Res Part A 2013:101A:1857–1864.
Polyethylene glycol–grafted polyethylenimine used to enhance adenovirus gene delivery†
Article first published online: 27 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 7, pages 1857–1864, July 2013
How to Cite
Singarapu, K., Pal, I. and Ramsey, J. D. (2013), Polyethylene glycol–grafted polyethylenimine used to enhance adenovirus gene delivery. J. Biomed. Mater. Res., 101A: 1857–1864. doi: 10.1002/jbm.a.34483
- Issue published online: 25 MAY 2013
- Article first published online: 27 NOV 2012
- Manuscript Accepted: 9 OCT 2012
- Manuscript Revised: 5 OCT 2012
- Manuscript Received: 17 AUG 2012
- gene delivery;
- hybrid vector;
An improved adenoviral-based gene delivery vector was developed by complexing adenovirus (Ad) with a biocompatible, grafted copolymer PEG-g-PEI composed of polyethylene glycol (PEG) and polyethylenimine (PEI). Although an Ad-based gene vector is considered relatively safe, its native tropism, tendency to elicit an immune response, and susceptibility to inactivating antibodies makes the virus less than ideal. The goal of the current study was to determine whether Ad could be complexed with a PEG-g-PEI copolymer that would enable the virus to transduce cells lacking the Ad receptor, while avoiding the issues commonly associated with PEI. A copolymer library was synthesized using 2 kDa PEG and either linear or branched PEI (25 kDa) with a PEG to PEI grafting ratio of 10, 20, or 30. The results of the study indicate that PEG-g-PEI/Ad complexes are indeed able to transduce CAR-negative NIH 3T3 cells. The results also demonstrate that the PEG-g-PEI/Ad complexes are less toxic, less hemolytic, and more appropriately sized than PEI/Ad complexes. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.