Get access

Incorporation of fibronectin to enhance cytocompatibility in multilayer elastin-like protein scaffolds for tissue engineering

Authors

  • Swathi Ravi,

    1. Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, Georgia 30332
    Search for more papers by this author
  • Jeffrey M. Caves,

    1. Department of Surgery, Emory University, Atlanta, Georgia 30322
    2. Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215
    3. Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02215
    Search for more papers by this author
  • Adam W. Martinez,

    1. Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, Georgia 30332
    Search for more papers by this author
  • Carolyn A. Haller,

    1. Department of Surgery, Emory University, Atlanta, Georgia 30322
    2. Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215
    3. Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02215
    Search for more papers by this author
  • Elliot L. Chaikof

    Corresponding author
    1. Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, Georgia 30332
    2. Department of Surgery, Emory University, Atlanta, Georgia 30322
    3. Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215
    4. Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02215
    • 110 Francis St, Suite 9F, Boston, MA 02215
    Search for more papers by this author

  • How to cite this article: Ravi S, Caves JM, Martinez AW, Haller CA, Chaikof EL. 2013. Incorporation of fibronectin to enhance cytocompatibility in multilayer elastin-like protein scaffolds for tissue engineering. J Biomed Mater Res Part A 2013:101A:1915–1925.

Abstract

Recombinant, elastin-like protein (ELP) polymers are of significant interest for the engineering of compliant, resilient soft tissues due to a wide range of tunable mechanical properties, biostability, and biocompatibility. Here, we enhance endothelial cell (EC) and mesenchymal stem cell compatibility with ELP constructs by addition of fibronectin (Fn) to the surface or bulk of ELP hydrogels. We find that cell adhesion, proliferation, and migration can be modulated by Fn addition. Adsorption of Fn to the hydrogel surface is more efficient than bulk blending. Surface immobilization of Fn by genipin crosslinking leads to stability without loss of bioactivity. Gels of varying mechanical modulus do not alter cell adhesion, proliferation, and migration in the range we investigate. However, more compliant gels promote an EC morphology suggesting tubulogenesis or network formation, whereas stiffer gels promote cobblestone morphology. Multilayer structures consisting of thin ELP sheets reinforced with collagen microfiber are fabricated and laminated through the culture of MSCs at layer interfaces. High cell viability in the resulting three-dimensional constructs suggests the applicability of Fn to the design of strong, resilient artificial blood vessels and other soft tissue replacements. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

Ancillary