These authors contributed equally to this work.
Development of a bone reconstruction technique using a solid free-form fabrication (SFF)-based drug releasing scaffold and adipose-derived stem cells†
Article first published online: 27 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 7, pages 1865–1875, July 2013
How to Cite
Lee, J. W., Kim, K.-J., Kang, K. S., Chen, S., Rhie, J.-W. and Cho, D.-W. (2013), Development of a bone reconstruction technique using a solid free-form fabrication (SFF)-based drug releasing scaffold and adipose-derived stem cells. J. Biomed. Mater. Res., 101A: 1865–1875. doi: 10.1002/jbm.a.34485
How to cite this article: Lee JW, Kim K-J, Kang KS, Chen S, Rhie J-W, Cho D-W. 2013. Development of a bone reconstruction technique using a solid free-form fabrication (SFF)-based drug releasing scaffold and adipose-derived stem cells. J Biomed Mater Res Part A 2013:101A:1865–1875.
- Issue published online: 25 MAY 2013
- Article first published online: 27 NOV 2012
- Manuscript Accepted: 9 OCT 2012
- Manuscript Revised: 8 OCT 2012
- Manuscript Received: 7 APR 2012
- National Research Foundation of Korea (NRF) grant by the Korea Government (MEST). Grant Number: 2012-0001235
- bone-tissue engineering;
- bone morphogenetic protein (BMP);
- human adipose-derived stem cells (hADSCs);
- solid free-form fabrication (SFF)
For tissue regeneration, three essential components of scaffolds, signals (biomolecules), and cells are required. Moreover, because bony defects are three-dimensional in many clinical circumstances, an exact 3D scaffold is important. Therefore, we proposed an effective reconstruction tool for cranial defects using human adipose-derived stem cells (hADSCs) and a 3D functional scaffold fabricated by solid free-form fabrication (SFF) technology that secretes biomolecules. We fabricated poly(propylene fumarate)-based 3D scaffolds with embedded microsphere-deliverable bone morphogenetic protein-2 (BMP-2) by microstereolithography. BMP-2-loaded SFF scaffolds with/without hADSCs (SFF/BMP/hADSCs scaffolds and SFF/BMP scaffolds, respectively) and BMP-2-unloaded SFF scaffolds (SFF scaffolds) were then implanted in rat crania, and in vivo bone formation was observed. Analyses of bone formation areas using micro-computed tomography (micro-CT) showed the superiority of SFF/BMP/hADSCs scaffolds. Hematoxylin and eosin stain, Masson's trichrome stain, and collagen type-I stain supported the results of the micro-CT scan. And human leukocyte antigen-ABC showed that seeded, differentiated hADSCs were well grown and changed to the bone tissue at the inside of the scaffold. Results showed that our combination of a functional 3D scaffold and hADSCs may be a useful tool for improving the reconstruction quality of severe bony defects in which thick bone is required. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.