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Development of a bone reconstruction technique using a solid free-form fabrication (SFF)-based drug releasing scaffold and adipose-derived stem cells

Authors

  • Jin Woo Lee,

    1. Department of NanoEngineering, The University of California, San Diego, La Jolla, California 92093-0448
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    • These authors contributed equally to this work.

  • Ki-Joo Kim,

    1. Department of Plastic Surgery, School of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea
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    • These authors contributed equally to this work.

  • Kyung Shin Kang,

    1. Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Gyungbuk, 790-784, Republic of Korea
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  • Shaochen Chen,

    1. Department of NanoEngineering, The University of California, San Diego, La Jolla, California 92093-0448
    2. Institute of Engineering in Medicine, The University of California, San Diego, La Jolla, California 92093-0448
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  • Jong-Won Rhie,

    Corresponding author
    1. Department of Plastic Surgery, School of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea
    • Department of Plastic Surgery, Catholic University College of Medicine, Seoul 137-701, Republic of Korea
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  • Dong-Woo Cho

    Corresponding author
    1. Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Gyungbuk, 790-784, Republic of Korea
    2. Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Gyungbuk, 790-784, Republic of Korea
    • Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja dong, Nam-gu, Pohang, Gyeongbuk 790-781, Republic of Korea
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  • How to cite this article: Lee JW, Kim K-J, Kang KS, Chen S, Rhie J-W, Cho D-W. 2013. Development of a bone reconstruction technique using a solid free-form fabrication (SFF)-based drug releasing scaffold and adipose-derived stem cells. J Biomed Mater Res Part A 2013:101A:1865–1875.

Abstract

For tissue regeneration, three essential components of scaffolds, signals (biomolecules), and cells are required. Moreover, because bony defects are three-dimensional in many clinical circumstances, an exact 3D scaffold is important. Therefore, we proposed an effective reconstruction tool for cranial defects using human adipose-derived stem cells (hADSCs) and a 3D functional scaffold fabricated by solid free-form fabrication (SFF) technology that secretes biomolecules. We fabricated poly(propylene fumarate)-based 3D scaffolds with embedded microsphere-deliverable bone morphogenetic protein-2 (BMP-2) by microstereolithography. BMP-2-loaded SFF scaffolds with/without hADSCs (SFF/BMP/hADSCs scaffolds and SFF/BMP scaffolds, respectively) and BMP-2-unloaded SFF scaffolds (SFF scaffolds) were then implanted in rat crania, and in vivo bone formation was observed. Analyses of bone formation areas using micro-computed tomography (micro-CT) showed the superiority of SFF/BMP/hADSCs scaffolds. Hematoxylin and eosin stain, Masson's trichrome stain, and collagen type-I stain supported the results of the micro-CT scan. And human leukocyte antigen-ABC showed that seeded, differentiated hADSCs were well grown and changed to the bone tissue at the inside of the scaffold. Results showed that our combination of a functional 3D scaffold and hADSCs may be a useful tool for improving the reconstruction quality of severe bony defects in which thick bone is required. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

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