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Poly-(L-lactic acid) and citric acid-crosslinked gelatin composite matrices as a drug-eluting stent coating material with endothelialization, antithrombogenic, and drug release properties

Authors

  • Motoki Inoue,

    1. Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS) 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
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  • Makoto Sasaki,

    1. Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS) 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
    2. Graduate School of Pure and Applied Science, University of Tsukuba, Japan
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  • Yasuyuki Katada,

    1. Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS) 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
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  • Katsuhito Fujiu,

    1. Department of Cardiovascular Medicine, the University of Tokyo, Graduate School of Medicine, Japan
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  • Ichiro Manabe,

    1. Department of Cardiovascular Medicine, the University of Tokyo, Graduate School of Medicine, Japan
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  • Ryozo Nagai,

    1. Department of Cardiovascular Medicine, the University of Tokyo, Graduate School of Medicine, Japan
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  • Tetsushi Taguchi

    Corresponding author
    1. Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS) 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
    2. Graduate School of Pure and Applied Science, University of Tsukuba, Japan
    • Graduate School of Pure and Applied Science, University of Tsukuba, Japan
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  • How to cite this article: Inoue M, Sasaki M, Katada Y, Fujiu K, Manabe I, Nagai R, Taguchi T. 2013. Poly-(L-lactic acid) and citric acid-crosslinked gelatin composite matrices as a drug-eluting stent coating material with endothelialization, antithrombogenic, and drug release properties. J Biomed Mater Res Part A 2013:101A:2049–2057.

Abstract

Biodegradable composite matrices comprising poly-(L-lactic acid) (PLLA) and citric acid-crosslinked alkali-treated gelatin (AlGelatin) with endothelialization, antithrombogenic, and drug release properties were prepared. The characterization of composite matrices with various mixing ratios was performed by evaluating their swelling ratio, endothelial cell culture, antithrombogenic tests, and drug release behavior. Tamibarotene (Am80), which specifically inhibits smooth muscle cell proliferation, was employed as the drug. The swelling ratio of composite matrices decreased as the PLLA content decreased. The number of endothelial cells cultured on the surfaces of composite matrices was maximal at the PLLA/AlGelatin-TSC ratio of 80/20. Antithrombogenic tests revealed that the levels of platelets and fibrin network formation decreased as the AlGelatin-TSC content increased. The Am80 release test indicated that the release rate decreased as PLLA content increased. Using the resulting composite matrix, Am80-eluting stents possessing a smooth surface and a coating thickness of ∼15 μm were successfully obtained. Am80 was continuously released from the resulting stent at ∼40%, up to 28 days without burst release. Therefore, Am80-eluting stent with its antithrombogenic and endothelialization properties has great potential for clinical use. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

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