How to cite this article: Zwingenberger S, Yao Z, Jacobi A, Vater C, Valladares RD, Li C, Nich C, Rao AJ, Christman JE, Antonios JK, Gibon E, Schambach A, Mätzig T, Günther K-P, Goodman SB, Stiehler M. 2013. Stem cell attraction via SDF-1α expressing fat tissue grafts. J Biomed Mater Res Part A 2013:101A:2067–2074.
Stem cell attraction via SDF-1α expressing fat tissue grafts†
Article first published online: 22 DEC 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 7, pages 2067–2074, July 2013
How to Cite
Zwingenberger, S., Yao, Z., Jacobi, A., Vater, C., Valladares, R. D., Li, C., Nich, C., Rao, A. J., Christman, J. E., Antonios, J. K., Gibon, E., Schambach, A., Mätzig, T., Günther, K.-P., Goodman, S. B. and Stiehler, M. (2013), Stem cell attraction via SDF-1α expressing fat tissue grafts. J. Biomed. Mater. Res., 101A: 2067–2074. doi: 10.1002/jbm.a.34512
- Issue published online: 25 MAY 2013
- Article first published online: 22 DEC 2012
- Manuscript Accepted: 24 OCT 2012
- Manuscript Revised: 8 OCT 2012
- Manuscript Received: 29 AUG 2012
- AO Foundation (Start up). Grant Number: S-10-67
- German Academic Exchange Service/Federal Ministry of Education and Research. Grant Number: D/09/04774
- Ellenburg Chair in Surgery, Stanford University
- MSC migration;
Mesenchymal stromal cell (MSCs) are key cellular components for site-specific tissue regeneration. The chemokine stromal derived factor 1 alpha (SDF-1α) is known to attract stem cells via the C-X-C chemokine receptor-4 (CXCR4) receptor. The aim of the study was to develop a model for stem cell attraction using SDF-1α overexpressing fat tissue grafts. Murine MSCs were lentiviral transduced to express the genes for enhanced green fluorescent protein, firefly luciferace, and human CXCR4 (hCXCR4). Murine fat tissue was adenoviral transduced to express SDF-1α and red fluorescent protein transgenes. MSCs were cultured on transwells with SDF-1α containing supernatants from transduced fat tissue. The numbers of migrated MSCs in four groups (with hCXCR4 positive (+) or hCXCR4 negative (−) MSCs with or without SDF-1α containing supernatant) were investigated. After 36 h of culture, 9025 ± 925 cells migrated through the membrane of the transwells in group 1 (CXCR4+/SDF-1α+), 4817 ± 940 cells in group 2 (CXCR4-/SDF-1α+), 2050 ± 766 cells in group 3 (CXCR4+/SDF-1α−), and 2108 ± 426 cells in group 4 (CXCR4-/SDF-1α-). Both, the presence of SDF-1α and the expression of hCXCR4 significantly increased the migration rates (p < 0.0001). MSCs overexpressing the CXCR4 receptor by lentiviral transduction are highly attracted by medium from SDF-1α expressing fat tissue in vitro. Thus, SDF-1α activated tissue grafts may be a strategy to enhance site-specific musculoskeletal tissue regeneration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.