3D interconnected porous HA scaffolds with SiO2 additions: Effect of SiO2 content and macropore size on the viability of human osteoblast cells

Authors

  • Jaru Nikom,

    1. Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
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  • Kanokwan Charoonpatrapong-Panyayong,

    1. Department of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
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  • Ureporn Kedjarune-Leggat,

    1. Department of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
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  • Ron Stevens,

    1. Department of Mechanical Engineering, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom
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  • Nudthakarn Kosachan,

    1. National Metal and Materials Technology Center, 114, Thailand Science Park, Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand
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  • Angkhana Jaroenworaluck

    Corresponding author
    1. National Metal and Materials Technology Center, 114, Thailand Science Park, Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand
    • National Metal and Materials Technology Center, 114, Thailand Science Park, Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand
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  • How to cite this article: Nikom J, Charoonpatrapong-Panyayong K, Kedjarune-Leggat U, Stevens R, Kosachan N, Jaroenworaluck A. 2013. 3D interconnected porous HA scaffolds with SiO2 additions: Effect of SiO2 content and macropore size on the viability of human osteoblast cells. J Biomed Mater Res Part A 2013:101A:2295–2305.

Abstract

3D interconnected porous scaffolds of HA and HA with various additions of SiO2 were fabricated using a polymeric template technique, to make bioceramic scaffolds consisting of macrostructures of the interconnected macropores. Three different sizes of the polyurethane template were used in the fabrication process to form different size interconnected macropores, to study the effect of pore size on human osteoblast cell viability. The template used allowed fabrication of scaffolds with pore sizes of 45, 60, and 75 ppi, respectively. Scanning microscopy was used extensively to observe the microstructure of the sintered samples and the characteristics of cells growing on the HA surfaces of the interconnected macropores. It has been clearly demonstrated that the SiO2 addition has influenced both the phase transformation of HA to TCP (β-TCP and α-TCP) and also affected the human osteoblast cell viability grown on these scaffolds. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

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