How to cite this article: Zhang X, Xu L, Wei S, Zhai M, Li J. 2013. Stimuli responsive deswelling of radiation synthesized collagen hydrogel in simulated physiological environment. J Biomed Mater Res Part A 2013:101A:2191–2201.
Stimuli responsive deswelling of radiation synthesized collagen hydrogel in simulated physiological environment †
Article first published online: 27 DEC 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 8, pages 2191–2201, August 2013
How to Cite
Zhang, X., Xu, L., Wei, S., Zhai, M. and Li, J. (2013), Stimuli responsive deswelling of radiation synthesized collagen hydrogel in simulated physiological environment . J. Biomed. Mater. Res., 101A: 2191–2201. doi: 10.1002/jbm.a.34525
- Issue published online: 23 JUN 2013
- Article first published online: 27 DEC 2012
- Manuscript Accepted: 13 NOV 2012
- Manuscript Revised: 2 NOV 2012
- Manuscript Received: 14 SEP 2012
- National Natural Science Foundation of China. Grant Number: 21103004
- National Key Technology R&D Program. Grant Number: 2011BAB02B05
- collagen hydrogel;
- radiation crosslinking;
- environmental stimuli response;
- tissue scaffold
Collagen hydrogels were prepared via radiation crosslinking. The simulated physiological environmental effects related to their biomedical applications on the volume phase transition of collagen hydrogel were studied, that is stimuli response to ions, temperature, and pH. The deswelling behavior of collagen hydrogel depends on the salt concentration, temperature, pH, and the hydrogel preparation procedure. Meanwhile, hydrogel structure related to the volume phase transition was investigated by FTIR, fluorescence spectrum, and HR-MAS NMR. Deswelling in salt solution caused little change on collagen conformation, and a denser network led to more significant tyrosine-derived fluorescence quenching. Hydrogen bonding between hydrated water and collagen polypeptide chain was dissociated and the activity of hydrophobic side chain increased, inducing a higher extent of contraction with the increasing of salt concentration. Moreover, salt solution treatments weakened the electrostatic interactions, side chains interactions, and hydrogen bonding of collagen hydrogel, which reduced the thermal stability of collagen hydrogel. Comparing with cell-free collagen hydrogel contraction, fibroblasts did not aggravate contraction of collagen hydrogel significantly. This study elucidated the deswelling mechanism of radiation crosslinked collagen hydrogel in simulated physiological environment and provides strategies for controlling the stimuli response of collagen hydrogel in biomedical application. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.