These authors contributed equally to this work.
Fabrication and characterization of regenerated silk scaffolds reinforced with natural silk fibers for bone tissue engineering†
Article first published online: 21 FEB 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 8, pages 2392–2404, August 2013
How to Cite
Mobini, S., Hoyer, B., Solati-Hashjin, M., Lode, A., Nosoudi, N., Samadikuchaksaraei, A. and Gelinsky, M. (2013), Fabrication and characterization of regenerated silk scaffolds reinforced with natural silk fibers for bone tissue engineering. J. Biomed. Mater. Res., 101A: 2392–2404. doi: 10.1002/jbm.a.34537
How to cite this article: Mobini S, Hoyer B, Solati-Hashjin M, Lode A, Nosoudi N, Samadikuchaksaraei A, Gelinsky M. 2013. Fabrication and characterization of regenerated silk scaffolds reinforced with natural silk fibers for bone tissue engineering. J Biomed Mater Res Part A 2013:101A:2392–2404.
- Issue published online: 23 JUN 2013
- Article first published online: 21 FEB 2013
- Manuscript Accepted: 13 NOV 2012
- Manuscript Revised: 11 NOV 2012
- Manuscript Received: 11 JUL 2012
- German Academic Exchange Service (DAAD, Germany)
- bone tissue engineering;
- natural silk fiber;
- human mesenchymal stem cells
We introduce a novel Bombyx mori silk-based composite material developed for bone tissue engineering. Three-dimensional scaffolds were fabricated by embedding of natural degummed silk fibers in a matrix of regenerated fibroin, followed by freeze-drying. Different ratios of fibers to fibroin were investigated with respect to their influence on mechanical and biological properties. For all scaffold types, an interconnected porous structure suitable for cell penetration was proven by scanning electron microscopy. Compressive tests, carried out in static and cyclic mode under dry as well as wet conditions, revealed a strong impact of fiber reinforcement on compressive modulus and compressive stress. Cell culture experiments with human mesenchymal stem cells demonstrated that the fiber/fibroin composite scaffolds support cell attachment, proliferation, as well as differentiation along the osteoblastic lineage. Considering the excellent mechanical and biological properties, novel fiber/fibroin scaffolds appear to be an interesting structure for prospect studies in bone tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2013.