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Fabrication and characterization of regenerated silk scaffolds reinforced with natural silk fibers for bone tissue engineering

Authors

  • Sahba Mobini,

    1. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
    2. Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus and Medical Faculty of Technische Universität Dresden, Dresden, Germany
    3. Department of Biomedical Engineering, Amir Kabir University of Technology, Tehran, Iran
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    • These authors contributed equally to this work.

  • Birgit Hoyer,

    1. Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus and Medical Faculty of Technische Universität Dresden, Dresden, Germany
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    • These authors contributed equally to this work.

  • Mehran Solati-Hashjin,

    Corresponding author
    1. Department of Biomedical Engineering, Amir Kabir University of Technology, Tehran, Iran
    • Department of Biomedical Engineering, Amir Kabir University of Technology, Tehran, Iran
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  • Anja Lode,

    1. Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus and Medical Faculty of Technische Universität Dresden, Dresden, Germany
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  • Nasim Nosoudi,

    1. Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus and Medical Faculty of Technische Universität Dresden, Dresden, Germany
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  • Ali Samadikuchaksaraei,

    1. Department of Medical Biotechnology, Cellular and Molecular Research Center, Tehran University of Medical Sciences, Tehran, Iran
    2. Department of Chemical Engineering, Biological Systems Engineering Laboratory, Imperial College London, London, United Kingdom
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  • Michael Gelinsky

    1. Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus and Medical Faculty of Technische Universität Dresden, Dresden, Germany
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  • How to cite this article: Mobini S, Hoyer B, Solati-Hashjin M, Lode A, Nosoudi N, Samadikuchaksaraei A, Gelinsky M. 2013. Fabrication and characterization of regenerated silk scaffolds reinforced with natural silk fibers for bone tissue engineering. J Biomed Mater Res Part A 2013:101A:2392–2404.

Abstract

We introduce a novel Bombyx mori silk-based composite material developed for bone tissue engineering. Three-dimensional scaffolds were fabricated by embedding of natural degummed silk fibers in a matrix of regenerated fibroin, followed by freeze-drying. Different ratios of fibers to fibroin were investigated with respect to their influence on mechanical and biological properties. For all scaffold types, an interconnected porous structure suitable for cell penetration was proven by scanning electron microscopy. Compressive tests, carried out in static and cyclic mode under dry as well as wet conditions, revealed a strong impact of fiber reinforcement on compressive modulus and compressive stress. Cell culture experiments with human mesenchymal stem cells demonstrated that the fiber/fibroin composite scaffolds support cell attachment, proliferation, as well as differentiation along the osteoblastic lineage. Considering the excellent mechanical and biological properties, novel fiber/fibroin scaffolds appear to be an interesting structure for prospect studies in bone tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2013.

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