Phenotypic redifferentiation and cell cluster formation of cultured human articular chondrocytes in a three-dimensional oriented gelatin scaffold in the presence of PGE2 - first results of a pilot study

Authors

  • Christoph Brochhausen,

    Corresponding author
    1. REPAIR-lab, European Institute of Excellence on Tissue Engineering & Regenerative Medicine, Institute of Pathology, Johannes Gutenberg-University, Langenbeckstraße 1, 55101 Mainz, Germany
    • REPAIR-lab, European Institute of Excellence on Tissue Engineering & Regenerative Medicine, Institute of Pathology, Johannes Gutenberg-University, Langenbeckstraße 1, 55101 Mainz, Germany
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    • These authors contributed equally to this work.

  • Natalia Sánchez,

    1. REPAIR-lab, European Institute of Excellence on Tissue Engineering & Regenerative Medicine, Institute of Pathology, Johannes Gutenberg-University, Langenbeckstraße 1, 55101 Mainz, Germany
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    • These authors contributed equally to this work.

  • Sven Halstenberg,

    1. REPAIR-lab, European Institute of Excellence on Tissue Engineering & Regenerative Medicine, Institute of Pathology, Johannes Gutenberg-University, Langenbeckstraße 1, 55101 Mainz, Germany
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  • Rolf Zehbe,

    1. Institute of Materials Science and Technologies, Technical University Berlin, Englische Straße 20, 10587 Berlin, Germany
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  • Bernhard Watzer,

    1. Department of Paediatrics, Philipps-University, Baldingerstraße, 35043 Marburg, Germany
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  • Volker H. Schmitt,

    1. REPAIR-lab, European Institute of Excellence on Tissue Engineering & Regenerative Medicine, Institute of Pathology, Johannes Gutenberg-University, Langenbeckstraße 1, 55101 Mainz, Germany
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  • Alexander Hofmann,

    1. Center for Trauma and Orthopaedic Surgery, Johannes Gutenberg-University, Langenbeckstraße 1, 55101 Mainz, Germany
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  • Andrea Meurer,

    1. Clinic of Special Orthopaedic Surgery, Orthopaedic Department Friedrichsheim gGmbH, Johann Wolfgang Goethe-University, Marienburgstrasse 2, 60528 Frankfurt, Germany
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  • Ron E. Unger,

    1. REPAIR-lab, European Institute of Excellence on Tissue Engineering & Regenerative Medicine, Institute of Pathology, Johannes Gutenberg-University, Langenbeckstraße 1, 55101 Mainz, Germany
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  • Charles James Kirkpatrick

    1. REPAIR-lab, European Institute of Excellence on Tissue Engineering & Regenerative Medicine, Institute of Pathology, Johannes Gutenberg-University, Langenbeckstraße 1, 55101 Mainz, Germany
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  • How to cite this article: Brochhausen C, Śnchez N, Halstenberg S, Zehbe R, Watzer B, Schmitt VH, Hofmann A, Meurer A, Unger RE, Kirkpatrick CJ. 2013. Phenotypic redifferentiation and cell cluster formation of cultured human articular chondrocytes in a three-dimensional oriented gelatin scaffold in the presence of PGE2 - first results of a pilot study. J Biomed Mater Res Part A 2013:101A:2374–2382.

Abstract

Modern tissue engineering strategies comprise three elemental parameters: cells, scaffolds and growth factors. Articular cartilage represents a highly specialized tissue which allows frictionless gliding of corresponding articulating surfaces. As the regenerative potential of cartilage is low, tissue engineering-based strategies for cartilage regeneration represent a huge challenge. Prostaglandins function as regulators in cartilage development and metabolism, especially in growth plate chondrocytes. In this study, it was analyzed if prostaglandin E2 (PGE2) has an effect on the phenotypic differentiation of human chondrocytes cultured in a three-dimensional (3D) gelatin-based scaffold made by directional freezing and subsequent freeze-drying. As a result, it was clearly demonstrated that low doses of PGE2 revealed beneficial effects on the phenotypic differentiation and collagen II expression of human articular chondrocytes in this 3D cell culture system. In conclusion, PGE2 is an interesting candidate for tissue engineering applications since it represents an already well-studied molecule which is available in pharmaceutical quality. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

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