How to cite this article: Kiang JD, Wen JH, del Álamo JC, Engler AJ. 2013. Dynamic and reversible surface topography influences cell morphology. J Biomed Mater Res Part A 2013:101A:2313–2321.
Dynamic and reversible surface topography influences cell morphology†
Article first published online: 27 JAN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 8, pages 2313–2321, August 2013
How to Cite
Kiang, J. D., Wen, J. H., del Álamo, J. C. and Engler, A. J. (2013), Dynamic and reversible surface topography influences cell morphology. J. Biomed. Mater. Res., 101A: 2313–2321. doi: 10.1002/jbm.a.34543
- Issue published online: 23 JUN 2013
- Article first published online: 27 JAN 2013
- Manuscript Accepted: 19 NOV 2012
- Manuscript Revised: 14 NOV 2012
- Manuscript Received: 20 AUG 2012
- National Institutes of Health. Grant Number: DP02OD006460
- San Diego Skeletal Muscle Research Center. Grant Number: P30AR061303
- magnetic field;
- cell area
Microscale and nanoscale surface topography changes can influence cell functions, including morphology. Although in vitro responses to static topography are novel, cells in vivo constantly remodel topography. To better understand how cells respond to changes in topography over time, we developed a soft polyacrylamide hydrogel with magnetic nickel microwires randomly oriented in the surface of the material. Varying the magnetic field around the microwires reversibly induced their alignment with the direction of the field, causing the smooth hydrogel surface to develop small wrinkles; changes in surface roughness, ΔRRMS, ranged from 0.05 to 0.70 μm and could be oscillated without hydrogel creep. Vascular smooth muscle cell morphology was assessed when exposed to acute and dynamic topography changes. Area and shape changes occurred when an acute topographical change was imposed for substrates exceeding roughness of 0.2 μm, but longer-term oscillating topography did not produce significant changes in morphology irrespective of wire stiffness. These data imply that cells may be able to use topography changes to transmit signals as they respond immediately to changes in roughness. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.