How to cite this article: Pati F, Kalita H, Adhikari B, Dhara S. 2013. Osteoblastic cellular responses on ionically crosslinked chitosan-tripolyphosphate fibrous 3-D mesh scaffolds. J Biomed Mater Res Part A 2013:00A:000–000.
Osteoblastic cellular responses on ionically crosslinked chitosan-tripolyphosphate fibrous 3-D mesh scaffolds†
Article first published online: 28 JAN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
How to Cite
Pati, F., Kalita, H., Adhikari, B. and Dhara, S. (2013), Osteoblastic cellular responses on ionically crosslinked chitosan-tripolyphosphate fibrous 3-D mesh scaffolds. J. Biomed. Mater. Res.. doi: 10.1002/jbm.a.34559
- Article first published online: 28 JAN 2013
- Manuscript Accepted: 27 NOV 2012
- Manuscript Revised: 21 NOV 2012
- Manuscript Received: 2 APR 2012
- ionic crosslinking;
- osteoblastic differentiation;
- bone tissue engineering
Tripolyphosphate (TPP) crosslinked chitosan (CH)-based fibrous matrices have potential as bioactive scaffolds for bone tissue engineering. This study describes mechanical, biomineralization, and in vitro bone cell growth and differentiation properties of CH-TPP (chitosan-tripolyphosphate) fibrous scaffolds and compared with that of uncrosslinked CH one. The hydrated CH-TPP scaffolds were viscoelastic in nature and their compressive strength was ∼2.9 MPa, which is greater than recent polymer experimental bone scaffolds. This improvement in mechanical properties of CH-TPP scaffold may be beneficial toward cancellous bone graft application. Furthermore, CH-TPP fibers supported in vitro biomineralization with phosphate as nucleation site; however, no significant difference in biomineralization morphology was observed with uncrosslinked CH fibers. Interestingly, a significant improvement in cellular responses (>33% increase in cell number based on DNA quantification) was observed when osteoblast like cells were cultured on the CH-TPP scaffolds than that of CH scaffolds without phosphate group. Enhanced osteoblastic differentiation of MG63 cells on CH-TPP scaffolds was also evidenced. Altogether, the results show that the CH-TPP fibrous scaffolds are encouraging for bone tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.