How to cite this article: Pati F, Kalita H, Adhikari B, Dhara S. 2013. Osteoblastic cellular responses on ionically crosslinked chitosan-tripolyphosphate fibrous 3-D mesh scaffolds. J Biomed Mater Res Part A 2013:00A:000–000.
1552-4965/asset/olbannerleft.jpg?v=1&s=343c79177ba7ba4a14e83022a135a85fff60897a)
1552-4965/asset/olbannerright.jpg?v=1&s=692ae6cfb9620aaa8486b8867aa2cc1f34ef54b2)
Osteoblastic cellular responses on ionically crosslinked chitosan-tripolyphosphate fibrous 3-D mesh scaffolds†
Article first published online: 28 JAN 2013
DOI: 10.1002/jbm.a.34559
Copyright © 2013 Wiley Periodicals, Inc.
Issue
Journal of Biomedical Materials Research Part A
Early View (Online Version of Record published before inclusion in an issue)
Additional Information
How to Cite
Pati, F., Kalita, H., Adhikari, B. and Dhara, S. (2013), Osteoblastic cellular responses on ionically crosslinked chitosan-tripolyphosphate fibrous 3-D mesh scaffolds. J. Biomed. Mater. Res.. doi: 10.1002/jbm.a.34559
- †
Publication History
- Article first published online: 28 JAN 2013
- Manuscript Accepted: 27 NOV 2012
- Manuscript Revised: 21 NOV 2012
- Manuscript Received: 2 APR 2012
- Abstract
- Article
- References
- Cited By
Keywords:
- chitosan-tripolyphosphate;
- ionic crosslinking;
- osteoconduction;
- osteoblastic differentiation;
- bone tissue engineering
Abstract
Tripolyphosphate (TPP) crosslinked chitosan (CH)-based fibrous matrices have potential as bioactive scaffolds for bone tissue engineering. This study describes mechanical, biomineralization, and in vitro bone cell growth and differentiation properties of CH-TPP (chitosan-tripolyphosphate) fibrous scaffolds and compared with that of uncrosslinked CH one. The hydrated CH-TPP scaffolds were viscoelastic in nature and their compressive strength was ∼2.9 MPa, which is greater than recent polymer experimental bone scaffolds. This improvement in mechanical properties of CH-TPP scaffold may be beneficial toward cancellous bone graft application. Furthermore, CH-TPP fibers supported in vitro biomineralization with phosphate as nucleation site; however, no significant difference in biomineralization morphology was observed with uncrosslinked CH fibers. Interestingly, a significant improvement in cellular responses (>33% increase in cell number based on DNA quantification) was observed when osteoblast like cells were cultured on the CH-TPP scaffolds than that of CH scaffolds without phosphate group. Enhanced osteoblastic differentiation of MG63 cells on CH-TPP scaffolds was also evidenced. Altogether, the results show that the CH-TPP fibrous scaffolds are encouraging for bone tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.