Cell toxicity of methacrylate monomers—The role of glutathione adduct formation
Article first published online: 24 APR 2013
Copyright © 2013 Wiley Periodicals, Inc., a Wiley Company
Journal of Biomedical Materials Research Part A
Volume 101, Issue 12, pages 3504–3510, December 2013
How to Cite
How to cite this article: Cell toxicity of methacrylate monomers— The role of glutathione adduct formation. J Biomed Mater Res Part A 2013:101A:3504–3510., , , . 2013.
- Issue published online: 28 OCT 2013
- Article first published online: 24 APR 2013
- Manuscript Accepted: 11 FEB 2013
- Manuscript Revised: 4 FEB 2013
- Manuscript Received: 2 JAN 2013
- dental restorative materials;
- methacrylate monomers;
- oxidative stress;
Polymer-based dental restorative materials are designed to polymerize in situ. However, the conversion of methacrylate monomer to polymer is never complete, and leakage of the monomer occurs. It has been shown that these monomers are toxic in vitro; hence concerns regarding exposure of patients and dental personnel have been raised. Different monomer methacrylates are thought to cause toxicity through similar mechanisms, and the sequestration of cellular glutathione (GSH) may be a key event. In this study we examined the commonly used monomer methacrylates, 2-hydroxyethylmethacrylate (HEMA), triethylenglycol-dimethacrylate (TEGDMA), bisphenol-A-glycidyl-dimethacrylate (BisGMA), glycerol-dimethacrylate (GDMA) and methyl-methacrylate (MMA). The study aimed to establish monomers' ability to complex with GSH, and relate this to cellular toxicity endpoints. Except for BisGMA, all the monomer methacrylates decreased the GSH levels both in cells and in a cell-free system. The spontaneous formation of methacrylate-GSH adducts were observed for all methacrylate monomers except BisGMA. However, we were not able to correlate GSH depletion and toxic response measured as SDH activity and changes in cell growth pattern. Together, the current study indicates mechanisms other than GSH-binding to be involved in the toxicity of methacrylate monomers. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3504–3510, 2013.