Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells
Article first published online: 26 AUG 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 102, Issue 2, pages 523–531, February 2014
How to Cite
How to cite this article: Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells. J Biomed Mater Res Part A 2014:102A:523–531., , , , . 2014.
- Issue published online: 14 DEC 2013
- Article first published online: 26 AUG 2013
- Accepted manuscript online: 27 MAR 2013 06:37AM EST
- Manuscript Accepted: 4 MAR 2013
- Manuscript Revised: 26 FEB 2013
- Manuscript Received: 9 JAN 2013
- hematopoietic cell adhesion;
- extracellular matrix;
- biomimetic scaffold
This study investigated the adhesive behaviors of normal and abnormal hematopoietic cells on nanotopographical materials. Previously, electrospun nanofiber scaffolds (NFSs) were used to capture and expand hematopoietic stem cells in vitro; here, we demonstrate that NFS could also serve as a useful bioadhesive platform for capturing functionally adherent leukemia cells. Collagen-blended poly(d,l-lactide-co-glycolide) NFS enabled more rapid and efficient capture of K562 leukemia cells than tissue culture polystyrene surfaces with up to 70% improved adhesion and shorter time. Cellular extensions, stronger adhesion, and enhanced cell–cell interactions were observed in K562 cells captured on NFS. While NFS promoted hematopoietic progenitor cell proliferation, it inhibited leukemia cell proliferation and affected cell cycle status by shifting more cells toward the G0/G1 phase. The expression of α-integrins was equally high in both captured and uncaptured leukemia cell populations demonstrating no relation to its adhesive nature. Hematopoietic morphological signatures of NFS captured cells presented no impact on cell differentiation. We conclude that electrospun NFS serves as an excellent platform not only for capturing functionally adherent leukemia cells but also for studying the impact of niche-like structure in the nanoscale. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 523–531, 2014.