Study of subcellular dynamics on cell–substrate interactions by live cell imaging
Article first published online: 19 JUL 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 102, Issue 4, pages 1149–1154, April 2014
How to Cite
How to cite this article: 2014. Study of subcellular dynamics on cell–substrate interactions by live cell imaging. J Biomed Mater Res Part A 2014:102A: 1149–1154., , .
- Issue published online: 21 FEB 2014
- Article first published online: 19 JUL 2013
- Accepted manuscript online: 29 APR 2013 10:50AM EST
- Manuscript Accepted: 22 APR 2013
- Manuscript Revised: 12 MAR 2013
- Manuscript Received: 23 JAN 2013
- National Science Council, Taiwan. Grant Number: 97-2221-E-027-099
- cell–substrate interaction;
- membrane expansion;
- live cell imaging;
- confocal microscopy
Cellular adhesiveness to biomaterial is one of the important properties for the success of tissue engineering. The cell–biomaterial interactions involve close cooperation of adhesion proteins, plasma membrane, and cytoskeletons to form focal adhesions during the process of anchoring. Dynamic development of the plasma membrane in the process reflects the cellular biocompatibility and motility. The process of cell attachment beginning from seeding, contact, attachment, and spreading has not been investigated. In this study, we monitored the whole process of cells attaching to the substrate surface by time-lapse confocal microscopy. We observed that the surface configuration of the substratum effects plasma membrane expansion and genomic materials distribution. In contrast to the cells grown on the plate, the cells attached on pillars are with rounded nuclei and with prominent lamellipodia spreading out. Membrane expansion is involved in dynamic development of the plasma membrane and lamellipodia formation for attachment, migration, or proliferation and reflects the cellular physiology status of the cells. This study provides a platform for investigation of cell behavior and dynamic development of subcellular structures regarding cell–biomaterial interactions. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1149–1154, 2014.