Preparation and properties of cellulose nanocrystals reinforced collagen composite films

Authors

  • Weichang Li,

    1. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, China
    2. Department of Chemistry, Jinan University, Guangzhou, China
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  • Rui Guo,

    1. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, China
    2. Department of Biomedical Engineering, Jinan University, Guangzhou, China
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  • Yong Lan,

    1. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, China
    2. Department of Materials Science and Engineering, Jinan University, Guangzhou, China
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  • Yi Zhang,

    1. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, China
    2. Department of Biomedical Engineering, Jinan University, Guangzhou, China
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  • Wei Xue,

    1. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, China
    2. Department of Biomedical Engineering, Jinan University, Guangzhou, China
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  • Yuanming Zhang

    1. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, China
    2. Department of Chemistry, Jinan University, Guangzhou, China
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  • These authors contributed equally to this work.

Correspondence to: Y. M. Zhang; e-mail: tzhangym@jnu.edu.cn

Abstract

Collagen films have been widely used in the field of biomedical engineering. However, the poor mechanical properties of collagen have limited its application. Here, rod-like cellulose nanocrystals (CNCs) were fabricated and used to reinforce collagen films. A series of collagen/CNCs films were prepared by collagen solution with CNCs suspensions homogeneously dispersed at CNCs: collagen weight ratios of 1, 3, 5, 7, and 10. The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing. Among the CNCs contents used, a loading of 7 wt % led to the maximum mechanical properties for the collagen/CNCs composite films. In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility. We conclude that the incorporation of CNCs is a simple and promising way to reinforce collagen films without impairing biocompatibility. This study demonstrates that the composite films show good potential for use in the field of skin tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1131–1139, 2014.

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