Influence of red algal sulfated polysaccharides on blood coagulation and platelets activation in vitro
Article first published online: 20 JUN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 102, Issue 5, pages 1431–1438, May 2014
How to Cite
How to cite this article: 2014. Influence of red algal sulfated polysaccharides on blood coagulation and platelets activation in vitro. J Biomed Mater Res Part A 2014: 102A:1431–1438., , , , , , .
- Issue published online: 24 MAR 2014
- Article first published online: 20 JUN 2013
- Accepted manuscript online: 13 JUN 2013 12:00AM EST
- Manuscript Accepted: 31 MAY 2013
- Manuscript Revised: 16 MAY 2013
- Manuscript Received: 24 JAN 2013
- Programs for Fundamental Research of the Presidium of Russian Academy of Sciences “Molecular-cell biology”, Presidium of Far-East Branch of the Russian Academy of Sciences
- platelet adhesion/aggregation;
- red blood cells
The influence of sulfated polysaccharides (λ-, κ-, and κ/β-carrageenan and porphyran) – on platelet activation was studied. Carrageenans were much weaker inhibitors of a coagulation process than heparin, while porphyran had not that effect. Results of the aPTT and PT assays suppose that carrageenans affected mostly intrinsic pathway of coagulation, while their effect on the extrinsic pathway is extremely low (λ and κ/β) or absent (κ, LMW derivative of κ-carrageenan). λ-Carrageenan was the most potent anticoagulant agent in TT, aPTT, PT, and anti-factor Xa activity. This sample was also the strongest inhibitor of collagen-induced platelet aggregation in PRP. Generally, the correlation of anticoagulant and antithrombotic action in PRP is preserved for carrageenans but not for heparin. Carrageenans and porphyran affected platelet adhesion to collagen by influencing glycoprotein VI. Low molecular weight κ-carrageenan had a similar effect on platelet adhesion mediated with both major collagen receptors: integrin α2β1 and glycoprotein VI as native polysaccharide had. Carrageenans resulted in activation of platelets under platelet adhesion mediated by integrin αIIbβ3 with less degree than heparin. The least sulfated κ/β-carrageenan that possessed an inhibiting effect on thrombin- and collagen-induced aggregation of washed platelets and on the PT test but it had no significant effect on TT was the weakest promoter of integrin αIIbβ3 mediated platelet activation. In summary, our study showed that the polysaccharide action was complex, since it depended on its molecular mass, sulfation degree, and monosaccharide contents (3,6-anhydrogalactose). © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1431–1438, 2014.