Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo
Article first published online: 15 JUL 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 102, Issue 6, pages 2055–2060, June 2014
How to Cite
How to cite this article: 2014. Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo. J Biomed Mater Res Part A 2014:102A:2055–2060., , , , , .
- Issue published online: 18 APR 2014
- Article first published online: 15 JUL 2013
- Accepted manuscript online: 13 JUN 2013 05:48PM EST
- Manuscript Accepted: 31 MAY 2013
- Manuscript Revised: 29 MAY 2013
- Manuscript Received: 22 JAN 2013
- Natural Science Foundation of Liaoning Province. Grant Number: 201102289
- Shenyang Municipal Science and Technology Projects. Grant Number: F12-193-9-12
- porous composite;
- foreign body response;
- implantable sensor
Chitosan (CS) is widely used as a scaffold material in tissue engineering. The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE). PCSM was fabricated with solvent casting/particulate leaching method using silica gel as porogen and characterized in vitro. Then, PCSM-Nafion and PCSM-PTFE composites were assembled with hydrated PCSM and implanted subcutaneously in rats. The histological analysis was performed in comparison with Nafion and PTFE. Implants were explanted 35, 65, and 100 days after the implantation. Histological assessments indicated that both composites achieved presumed effects of porous coatings on decreasing collagen deposition and promoting angiogenesis. PCSM-PTFE exerted higher collagen deposition by area ratio, both within and outside, compared with that of PCSM-Nafion. Angiogenesis within and outside the PCSM-Nafion both increased over time, but that of the PCSM-PTFE within decreased. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2055–2060, 2014.