In vivo study of the biocompatibility of a novel compressed collagen hydrogel scaffold for artificial corneas

Authors

  • Xianghua Xiao,

    1. Shaanxi Institute of Ophthalmology, Xi'an, Shaanxi, People's Republic of China
    2. Shaanxi Key Laboratory of Eye, Xi'an, Shaanxi, People's Republic of China
    3. Xi'an First Hospital, Xi'an, Shaanxi, People's Republic of China
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  • Shiyin Pan,

    1. Shaanxi Institute of Ophthalmology, Xi'an, Shaanxi, People's Republic of China
    2. Shaanxi Key Laboratory of Eye, Xi'an, Shaanxi, People's Republic of China
    3. Xi'an First Hospital, Xi'an, Shaanxi, People's Republic of China
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  • Xianning Liu,

    1. Shaanxi Institute of Ophthalmology, Xi'an, Shaanxi, People's Republic of China
    2. Shaanxi Key Laboratory of Eye, Xi'an, Shaanxi, People's Republic of China
    3. Xi'an First Hospital, Xi'an, Shaanxi, People's Republic of China
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  • Xiuping Zhu,

    1. Shaanxi Institute of Ophthalmology, Xi'an, Shaanxi, People's Republic of China
    2. Shaanxi Key Laboratory of Eye, Xi'an, Shaanxi, People's Republic of China
    3. Xi'an First Hospital, Xi'an, Shaanxi, People's Republic of China
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  • Che John Connon,

    1. School of Pharmacy, University of Reading, Reading, United Kingdom
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  • Jie Wu,

    1. Xi'an First Hospital, Xi'an, Shaanxi, People's Republic of China
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  • Shengli Mi

    Corresponding author
    1. Biomanufacturing Engineering Laboratory, Graduate School at Shenzhen, Tsinghua University, Shenzhen, People's Republic of China
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Abstract

The experiments were designed to evaluate the biocompatibility of a plastically compressed collagen scaffold (PCCS). The ultrastructure of the PCCS was observed via scanning electron microscopy. Twenty New Zealand white rabbits were randomly divided into experimental and control groups that received corneal pocket transplantation with PCCS and an amniotic membrane, respectively. And the contralateral eye of the implanted rabbit served as the normal group. On the 1st, 7th, 14th, 21st, 30th, 60th, 90th, and 120th postoperative day, the eyes were observed via a slit lamp. On the 120th postoperative day, the rabbit eyes were enucleated to examine the tissue compatibility of the implanted stroma. The PCCS was white and translucent. The scanning electron microscopy results showed that fibers within the PCCS were densely packed and evenly arranged. No edema, inflammation, or neovascularization was observed on ocular surface under a slit lamp and few lymphocytes were observed in the stroma of rabbit cornea after histological study. In conclusion, the PCCS has extremely high biocompatibility and is a promising corneal scaffold for an artificial cornea. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1782–1787, 2014.

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