Sericin–carboxymethyl cellulose porous matrices as cellular wound dressing material
Article first published online: 30 JUL 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 102, Issue 6, pages 1928–1940, June 2014
How to Cite
How to cite this article: 2014. Sericin–carboxymethyl cellulose porous matrices as cellular wound dressing material. J Biomed Mater Res Part A 2014;102A:1928–1940., .
- Issue published online: 18 APR 2014
- Article first published online: 30 JUL 2013
- Accepted manuscript online: 12 JUL 2013 08:48PM EST
- Manuscript Accepted: 25 JUN 2013
- Manuscript Revised: 22 MAY 2013
- Manuscript Received: 12 APR 2013
- Department of Biotechnology and its Bioinformatics facilities also fellowship to SN, Department of Science and Technology, Government of India, New Delhi, India
- Antheraea mylitta;
- growth factor;
- wound dressing
In this study, porous three-dimensional (3D) hydrogel matrices are fabricated composed of silk cocoon protein sericin of non-mulberry silkworm Antheraea mylitta and carboxymethyl cellulose. The matrices are prepared via freeze-drying technique followed by dual cross-linking with glutaraldehyde and aluminum chloride. The microstructure of the hydrogel matrices is assessed using scanning electron microscopy and biophysical characterization are carried out using Fourier transform infrared spectroscopy and X-ray diffraction. The transforming growth factor β1 release from the cross-linked matrices as a growth factor is evaluated by immunosorbent assay. Live dead assay and 3-[4,5-dimethylthiazolyl-2]-2,5-diphenyl tetrazolium bromide assay show no cytotoxicity of blended matrices toward human keratinocytes. The matrices support the cell attachment and proliferation of human keratinocytes as observed through scanning electron microscope and confocal images. Gelatin zymography demonstrates the low levels of matrix metalloproteinase 2 (MMP-2) and insignificant amount of MMP-9 in the culture media of cell seeded matrices. Low inflammatory response of the matrices is indicated through tumor necrosis factor alpha release assay. The results indicate that the fabricated matrices constitute 3D cell-interactive environment for tissue engineering applications and its potential use as a future cellular biological wound dressing material. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.