Photoinitiated chemical vapor deposition of cytocompatible poly(2-hydroxyethyl methacrylate) films
Article first published online: 10 SEP 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 102, Issue 7, pages 2375–2382, July 2014
How to Cite
How to cite this article: 2014. Photoinitiated chemical vapor deposition of cytocompatible poly(2-hydroxyethyl methacrylate) films. J Biomed Mater Res Part A 2014: 102A: 2375–2382., , , , , .
- Issue published online: 23 MAY 2014
- Article first published online: 10 SEP 2013
- Accepted manuscript online: 12 JUL 2013 08:39PM EST
- Manuscript Accepted: 3 JUL 2013
- Manuscript Revised: 20 JUN 2013
- Manuscript Received: 22 JAN 2013
- National Science Foundation. Grant Number: 0727984
- photoinitiated chemical vapor deposition;
- cell culture coating;
Poly(2-hydroxyethyl methacrylate) (pHEMA) is a widely utilized biomaterial due to lack of toxicity and suitable mechanical properties; conformal thin pHEMA films produced via chemical vapor deposition (CVD) would thus have broad biomedical applications. Thin films of pHEMA were deposited using photoinitiated CVD (piCVD). Incorporation of ethylene glycol diacrylate (EGDA) into the pHEMA polymer film as a crosslinker, confirmed via Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, resulted in varied swelling and degradation behavior. 2-Hydroxyethyl methacrylate-only films showed significant thickness loss (up to 40%), possibly due to extraction of low-molecular-weight species or erosion, after 24 h in aqueous solution, whereas films crosslinked with EGDA (9.25–12.4%) were stable for up to 21 days. These results differ significantly from those obtained with plasma-polymerized pHEMA, which degraded steadily over a 21-day period, even with crosslinking. This suggests that the piCVD films differ structurally from those fabricated via plasma polymerization (plasma-enhanced CVD). piCVD pHEMA coatings proved to be good cell culture materials, with Caco-2 cell attachment and viability comparable to results obtained on tissue-culture polystyrene. Thus, thin film CVD pHEMA offers the advantage of enabling conformal coating of a cell culture substrate with tunable properties depending on method of preparation and incorporation of crosslinking agents. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2375–2382, 2014.