Development of an elastic cell culture substrate for a novel uniaxial tensile strain bioreactor
Article first published online: 31 AUG 2013
© 2013 The Authors. Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Journal of Biomedical Materials Research Part A
Volume 102, Issue 7, pages 2356–2364, July 2014
How to Cite
How to cite this article: 2014. Development of an elastic cell culture substrate for a novel uniaxial tensile strain bioreactor. J Biomed Mater Res Part A 2014: 102A: 2356–2364., , .
- Issue published online: 23 MAY 2014
- Article first published online: 31 AUG 2013
- Accepted manuscript online: 14 AUG 2013 08:07AM EST
- Manuscript Accepted: 9 AUG 2013
- Manuscript Revised: 30 JUL 2013
- Manuscript Received: 7 JUN 2013
- EPSRC. Grant Number: EP/P505658/1
- surface modification;
Bioreactors can be used for mechanical conditioning and to investigate the mechanobiology of cells in vitro. In this study a polyurethane (PU), Chronoflex AL, was evaluated for use as a flexible cell culture substrate in a novel bioreactor capable of imparting cyclic uniaxial tensile strain to cells. PU membranes were plasma etched, across a range of operating parameters, in oxygen. Contact angle analysis and X-ray photoelectron spectroscopy showed increases in wettability and surface oxygen were related to both etching power and duration. Atomic force microscopy demonstrated that surface roughness decreased after etching at 20 W but was increased at higher powers. The etching parameters, 20 W 40 s, produced membranes with high surface oxygen content (21%), a contact angle of 66° ± 7° and reduced topographical features. Etching and protein conditioning membranes facilitated attachment, and growth to confluence within 3 days, of MG-63 osteoblasts. After 2 days with uniaxial strain (1%, 30 cycles/min, 1500 cycles/day), cellular alignment was observed perpendicular to the principal strain axis, and found to increase after 24 h. The results indicate that the membrane supports culture and strain transmission to adhered cells. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2356–2364, 2014.