†These authors contributed equally to this work.
Ultrasound-assisted fabrication of a biocompatible magnetic hydroxyapatite
Article first published online: 12 DEC 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 102, Issue 10, pages 3704–3712, October 2014
How to Cite
How to cite this article: 2014. Ultrasound-assisted fabrication of a biocompatible magnetic hydroxyapatite. J Biomed Mater Res Part A 2014:102A:3704–3712., , , , , .
- Issue published online: 23 AUG 2014
- Article first published online: 12 DEC 2013
- Accepted manuscript online: 29 NOV 2013 03:42AM EST
- Manuscript Accepted: 18 NOV 2013
- Manuscript Revised: 30 OCT 2013
- Manuscript Received: 25 SEP 2013
- National Basic Research Program of China
- 2011CB710901Contract grant sponsor: NSFC
- 11002016, 61227902, 10925208, 11120101001Contract grant sponsor: National Basic Research Program of China
- 2011CB710901Contract grant sponsor: National Key Technology R&D Program
- 2012BAI18B06, 2012BAI18B05Contract grant sponsor: 111 Project of China . Grant Number: B13003Contract grant sponsors: International Joint Research Center of Aerospace Biotechnology and Medical Engineering, Ministry of Science and Technology of China, and the Fundamental Research Funds for the Central Universities of China
This work describes the fabrication and characterization of a biocompatible magnetic hydroxyapatite (HA) using an ultrasound-assisted co-precipitation method. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM) were used to characterize the structure and chemical composition of the produced samples. The M–H loops of synthesized materials were traced using a vibrating sample magnetometer (VSM) and the biocompatibility was evaluated by cell culture and MTT (3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide) assay. Furthermore, in vivo histopathological examinations were used to evaluate the potential toxicological effects of Fe3O4-HA composites on kidney of SD rats injected intraperitoneally with Fe3O4-HA particles. The results showed that magnetic iron oxide particles first replace OH ions of HA, which are parallel to the c axis, and then enter the HA crystal lattice which produces changes in the crystal surface of HA. Chemical bond interaction was observed between PO43− groups of HA and iron ions of Fe3O4. The saturation magnetization (MS) of Fe3O4-HA composites was 46.36 emu/g obtained from VSM data. Cell culture and MTT assays indicated that HA could affect the growth and proliferation of HEK-293 cells. This Fe3O4-HA composite produced no negative effects on cell morphology, viability, and proliferation and exhibited remarkable biocompatibility. Moreover, no inflammatory cell infiltration was observed in kidney histopathology slices. Therefore, this study succeeds to develop a Fe3O4-HA composite as a prospective biomagnetic material for future applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3704–3712, 2014.