Electrospinning of gelatin fibers and gelatin/PCL composite fibrous scaffolds

Authors

  • Yanzhong Zhang,

    Corresponding author
    1. Division of Bioengineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576
    2. Department of Mechanical Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576
    • Division of Bioengineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576
    Search for more papers by this author
  • Hongwei Ouyang,

    1. Department of Orthopedic Surgery, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119274
    Search for more papers by this author
  • Chwee Teck Lim,

    1. Division of Bioengineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576
    2. Department of Mechanical Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576
    3. Nanoscience and Nanotechnology Initiative, National University of Singapore, 9 Engineering Drive 1, Singapore 117576
    Search for more papers by this author
  • Seeram Ramakrishna,

    1. Division of Bioengineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576
    2. Department of Mechanical Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576
    3. Nanoscience and Nanotechnology Initiative, National University of Singapore, 9 Engineering Drive 1, Singapore 117576
    Search for more papers by this author
  • Zheng-Ming Huang

    1. School of Aeronautics, Astronautics & Mechanics, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China
    Search for more papers by this author

Abstract

In this article, ultrafine gelatin (Gt) fibers were successfully produced with the use of the electrical spinning or electrospinning technique. A fluorinated alcohol of 2,2,2-trifluoroethanol (TFE) was used as the dissolving solvent. The morphology of the electrospun gelatin fibers was found to be dependent on the alteration of gelatin concentration ranging from 2.5% w/v to 12.5% w/v at 2.5% increment intervals. Based on the electrospun gelatin fibers obtained, 10% w/v gelatin/TFE solution was selected and mixed with 10% w/v poly(ϵ-caprolactone) (PCL) in TFE at a ratio of 50:50 and co-electrospun to produce gelatin/PCL composite membranes. Contact-angle measurement and tensile tests indicated that the gelatin/PCL complex fibrous membrane exhibited improved mechanical properties as well as more favorable wettability than that obtained from either gelatin or PCL alone. The gelatin/PCL fibrous membranes were further investigated as a promising scaffold for bone-marrow stromal cell (BMSC) culture. Scanning electron microscopy (SEM) and laser confocal microscopy observations showed that the cells could not only favorably attach and grow well on the surface of these scaffolds, but were also able to migrate inside the scaffold up to 114 μm within 1 week of culture. These results suggest the potential of using composite gelatin/PCL fibrous scaffolds for engineering three-dimensional tissues. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 72B: 156–165, 2005

Ancillary