Get access

Changes in stiffness of demineralized dentin following application of collagen crosslinkers

Authors

  • Ana Karina B. Bedran-Russo,

    Corresponding author
    1. Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, Chicago, Illinois
    • Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, Chicago, Illinois
    Search for more papers by this author
  • David H. Pashley,

    1. Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, Georgia
    Search for more papers by this author
  • Kelli Agee,

    1. Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, Georgia
    Search for more papers by this author
  • James L. Drummond,

    1. Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, Chicago, Illinois
    Search for more papers by this author
  • Klaus J. Miescke

    1. Department of Mathematics, Statistics, and Computer Science, University of Illinois at Chicago, Chicago, Illinois
    Search for more papers by this author

Abstract

It is thought that increasing the strength of the dentin matrix using crosslinking agents may improve both the strength and the durability of resin–dentin bonds. The purpose of this study was to evaluate the effect of two collagen crosslinking agents (glutaraldehyde, GD and grape seed extract, GSE) on the modulus of elasticity of demineralized dentin. Sound molar fragments were fully demineralized and divided into five groups according to the type and concentration of crosslinking agents: 2.5% GD; 5% GD, 25% GD; 0.65% GSE; 6.5% GSE. Specimens were immersed in their respective solution and tested at baseline, 10 min, 30 min, 1 h, 2 h, 4 h. The elastic modulus of dentin was significantly affected by the treatment (p < 0.01) and exposure time (p < 0.01). There was a statistically significant interaction between the two factors evaluated (treatment vs. time p < 0.01). Mean baselines values varied between 4.8 and 6.2 MPa in water; after 4 h of treatment the values increased between 34.9 and 242.5 MPa, that were treatment time and agent dependent. The use of these collagen crosslinkers to increase the stiffness of demineralized dentin, was both concentration and time dependent. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008

Ancillary