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The role of hydroxyapatite as solid signal on performance of PCL porous scaffolds for bone tissue regeneration

Authors

  • Vincenzo Guarino,

    1. Institute of Composite and Biomedical Materials, National Research Council and CRIB, University of Naples “Federico II” Piazzale Tecchio 80, 80125 Naples, Italy
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  • Filippo Causa,

    Corresponding author
    1. Department of Experimental and Clinical Medicine, University of Magna Graecia, Germaneto, 88100 Catanzaro, Italy
    • Department of Experimental and Clinical Medicine, University of Magna Graecia, Germaneto, 88100 Catanzaro, Italy
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  • Paolo A. Netti,

    1. Institute of Composite and Biomedical Materials, National Research Council and CRIB, University of Naples “Federico II” Piazzale Tecchio 80, 80125 Naples, Italy
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  • Gabriela Ciapetti,

    1. Laboratory for Pathophysiology of Orthopaedic Implants, Istituti Ortopedici Rizzoli, via di Barbiano 1/10, 40136 Bologna, Italy
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  • Stefania Pagani,

    1. Laboratory for Pathophysiology of Orthopaedic Implants, Istituti Ortopedici Rizzoli, via di Barbiano 1/10, 40136 Bologna, Italy
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  • Desiree Martini,

    1. Department of Human Anatomy, University of Bologna, via Irnerio 48, 40126 Bologna, Italy
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  • Nicola Baldini,

    1. Laboratory for Pathophysiology of Orthopaedic Implants, Istituti Ortopedici Rizzoli, via di Barbiano 1/10, 40136 Bologna, Italy
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  • Luigi Ambrosio

    1. Institute of Composite and Biomedical Materials, National Research Council and CRIB, University of Naples “Federico II” Piazzale Tecchio 80, 80125 Naples, Italy
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Abstract

Highly porous composites made up of biodegradable poly-ε-caprolactone (PCL) and stoichiometric hydroxyapatite (HA) particles have been developed as substrate for bone-tissue regeneration. The processing technique consists of phase inversion and particulate (salt crystals) leaching. Three different HA contents (13, 20 and 26 vol %) in PCL-based composite were considered in this study. Pore microstructure with fully interconnected network and pore sizes ranging around a few hundred of μm (macroporosity) was obtained as a result of salt particles removal by leaching process. Several microns (microporosity) porosity was also created through phase inversion of polymer solution. Total porosity up to 95% was achieved. Human marrow stromal cells (MSC) were seeded onto porous PCL-based composites for 1–5 weeks and cultured in osteogenic medium. MSC were able to adhere and grow on PCL-based substrates with a plateau at 3–4 weeks. However, the small effect of bioactive signals on the biological response evaluated in MSC cell culture suggests a prior role of topography on the biological response. Importantly, the presence of HA as a bioactive solid signal determines an increase of mechanical properties. On the overall, the results indicated that porous PCL-based composites are potential candidate for bone substitution with beneficial influence on structural characteristics by solid signal addition. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2008

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